YIpMELalpha2 vector (V001473)

Basic Vector Information

      • Vector Name:
      • YIpMELalpha2
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6716 bp
      • Type:
      • UAS-less reporter vector
      • Source/Author:
      • Melcher K, Sharma B, Ding WV, Nolden M.

YIpMELalpha2 vector Vector Map

YIpMELalpha26716 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600MEL1UAS deleted MEL1 promoterM13 fwdURA3URA3 promoterAmpR promoterAmpRoriCAP binding sitelac promoterlac operator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YIpMELalpha2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_49772        6716 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UAS-less reporter vector YIpMELalpha2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6716)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Zero background yeast reporter plasmids
  JOURNAL   Gene 247 (1-2), 53-61 (2000)
  PUBMED    10773444
REFERENCE   2  (bases 1 to 6716)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2003) Institute for Microbiology, Frankfurt 
            University, Marie Curie Strasse 9, Frankfurt 60439, Germany
REFERENCE   3  (bases 1 to 6716)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6716)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 247 
            (1-2), 53-61 (2000)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-SEP-2003) Institute for Microbiology, Frankfurt University, 
            Marie Curie Strasse 9, Frankfurt 60439, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(413..1825)
                     /gene="MEL1"
                     /label=MEL1
                     /note="Alpha-galactosidase 1 from Saccharomyces cerevisiae.
                     Accession#: P04824"
     regulatory      complement(1826..2478)
                     /label=UAS deleted MEL1 promoter
                     /note="UAS deleted MEL1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(2488..2504)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2780..3580)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(3581..3791)
                     /label=URA3 promoter
     promoter        4595..4699
                     /label=AmpR promoter
     CDS             4700..5557
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5731..6319
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    6607..6628
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6643..6673
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6681..6697
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

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