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yGALset985 vector

Basic Vector Information

Basic Vector Information
Vector Name yGALset985 Antibiotic Resistance Ampicillin
Length 5973 bp Type Cloning vector
Source Enomoto S, Chen G, Berman J.

yGALset985 vector Vector Map

yGALset985 vector Sequence

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LOCUS       Exported                5973 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector yGALset985, complete sequence.
ACCESSION   AF041807
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Enomoto S, Chen G, Berman J.
  TITLE     Vectors for expressing T7 epitope- and His6 affinity-tagged fusion 
            proteins in S. cerevisiae
  JOURNAL   BioTechniques 24 (5), 782-786 (1998)
  PUBMED    9591127
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Enomoto S, Berman J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-1998) Plant Biology, University of Minnesota, 220 
            Biological Sciences Center, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 5973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Cloning vector yGALset985"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:71554"
     promoter        70..88
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      112..793
                     /regulatory_class="promoter"
                     /note="S. cerevisiae GAL10 promoter"
     promoter        121..785
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    452..569
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     misc_feature    794..929
                     /label=5' recombination region
                     /note="5' recombination region"
     RBS             804..826
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    846..863
                     /label=polyhistidine affinity tag
                     /note="polyhistidine affinity tag"
     CDS             846..863
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    867..899
                     /label=T7 tag epitope
                     /note="T7 tag epitope"
     CDS             867..899
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             903..926
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      912..926
                     /regulatory_class="other"
                     /note="enterokinase recognition sequence"
     misc_feature    926..981
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     misc_feature    973..1178
                     /label=3' recombination region
                     /note="3' recombination region"
     terminator      1036..1083
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1238..2830
                     /label=S. cerevisiae 2 micron plasmid STB and ARS region
                     /note="S. cerevisiae 2 micron plasmid STB and ARS region"
     rep_origin      1475..2821
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     gene            complement(2854..3961)
                     /gene="ura3"
                     /label=ura3
                     /note="S. cerevisiae URA3"
     CDS             complement(2932..3735)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3736..3951)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     promoter        4067..4171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4172..5032
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="ampicillin resistance gene (apr/amp); E. coli 
                     beta-lactamase gene"
                     /protein_id="AAC40222.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5203..5791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      5785
                     /label=pUC origin
                     /note="pUC origin"

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