Basic Vector Information
yGALset352 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
yGALset352 vector Sequence
LOCUS 40924_49702 6345 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector yGALset352, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6345)
AUTHORS Enomoto S, Chen G, Berman J.
TITLE Vectors for expressing T7 epitope- and His6 affinity-tagged fusion
proteins in S. cerevisiae
JOURNAL BioTechniques 24 (5), 782-786 (1998)
PUBMED 9591127
REFERENCE 2 (bases 1 to 6345)
AUTHORS Enomoto S, Berman J.
TITLE Direct Submission
JOURNAL Submitted (09-JAN-1998) Plant Biology, University of Minnesota, 220
Biological Sciences Center, St. Paul, MN 55108, USA
REFERENCE 3 (bases 1 to 6345)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6345)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"BioTechniques"; date: "1998"; volume: "24"; issue: "5"; pages:
"782-786"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(09-JAN-1998) Plant Biology, University of Minnesota, 220 Biological
Sciences Center, St. Paul, MN 55108, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6345
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 70..88
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(121..785)
/label=GAL1,10 promoter
/note="divergent inducible promoter, regulated by Gal4"
RBS 804..826
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 846..863
/codon_start=1
/label=6xHis
/note="6xHis affinity tag"
/translation="HHHHHH"
CDS 867..899
/codon_start=1
/label=T7 tag (gene 10 leader)
/note="leader peptide from bacteriophage T7 gene 10"
/translation="MASMTGGQQMG"
CDS 903..926
/codon_start=1
/label=Xpress(TM) tag
/note="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/translation="DLYDDDDK"
misc_feature 936..982
/label=multiple cloning sites
/note="multiple cloning sites"
terminator 1046..1093
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
misc_feature complement(1807..2969)
/label=CEN/ARS
/note="S. cerevisiae CEN4 centromere fused to the
autonomously replicating sequence ARS1/ARS416"
CDS complement(3307..4107)
/codon_start=1
/label=URA3
/note="orotidine-5'-phosphate decarboxylase, required for
uracil biosynthesis"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(4108..4323)
/label=URA3 promoter
promoter 4439..4543
/label=AmpR promoter
CDS 4544..5401
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5575..6163
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
This page is informational only.