Basic Vector Information
- Vector Name:
- YFV-C2opt
- Antibiotic Resistance:
- Ampicillin
- Length:
- 10928 bp
- Type:
- VEEV replicon vector
- Replication origin:
- ori
- Source/Author:
- Shustov AV, Frolov IV.
YFV-C2opt vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
YFV-C2opt vector Sequence
LOCUS 40924_49682 10928 bp DNA circular SYN 18-DEC-2018
DEFINITION VEEV replicon vector YFV-C2opt, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10928)
AUTHORS Shustov AV, Frolov IV.
TITLE Trans-packaging of Yellow Fever virus replicons: an approach for
flaviviral antigen production
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10928)
AUTHORS Shustov AV, Frolov IV.
TITLE Direct Submission
JOURNAL Submitted (10-DEC-2005) Department of Microbiology and Immunology,
University of Texas Medical Branch, 301 University Boulevard,
Galveston, TX 77550-1019, USA
REFERENCE 3 (bases 1 to 10928)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 10928)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-DEC-2005) Department of Microbiology and Immunology, University
of Texas Medical Branch, 301 University Boulevard, Galveston, TX
77550-1019, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..10928
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 2..45
/note="5'UTR of Venezuelan equine encephalitis virus VEE
TC83"
misc_feature 46..1650
/note="nsP1 of Venezuelan equine encephalitis virus VEE
TC83"
RBS 1536..1544
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
misc_feature 1651..4032
/note="nsP2 of Venezuelan equine encephalitis virus VEE
TC83"
misc_feature 4033..5703
/note="nsP3 of Venezuelan equine encephalitis virus VEE
TC83"
misc_feature 5704..7524
/note="nsP4 of Venezuelan equine encephalitis virus VEE
TC83"
misc_feature 7502..7546
/note="subgenomic promoter of Venezuelan equine
encephalitis virus VEE TC83"
misc_feature 7571..7933
/note="Yellow fever virus 17D capsid protein (synthetic,
codon-optimized)"
misc_feature 7934..7993
/note="first 20 aa from PR of Yellow fever virus 17D
(synthetic, codon-optimized)"
misc_feature 7997..8041
/note="subgenomic promoter of Venezuelan equine
encephalitis virus VEE TC83 (second copy)"
CDS 8071..8667
/label=PuroR
/note="puromycin N-acetyltransferase"
misc_feature 8747..9102
/note="3'UTR of Venezuelan equine encephalitis virus VEE
TC83"
promoter 9166..9270
/label=AmpR promoter
CDS 9271..10128
/label=AmpR
/note="beta-lactamase"
rep_origin 10302..10890
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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