YEp24PGK vector (V001491)

LOCUS       40924_49537        9637 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YEp24PGK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Johansson B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2013) Department of Biology, University of Minho, 
            Campus de Gualtar, Braga 4710-051, Portugal
REFERENCE   2  (bases 1 to 9637)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9637)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (01-FEB-2013) Department of Biology, University of Minho, Campus de 
            Gualtar, Braga 4710-051, Portugal"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      348..1690
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2286..2506
                     /label=URA3 promoter
     CDS             2507..3307
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      3452..4934
                     /label=from Saccharomyces cerevisiae PGK1
                     /note="from Saccharomyces cerevisiae PGK1"
                     /regulatory_class="promoter"
     terminator      5030..5300
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             5363..6550
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             7194..7382
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7487..7627
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7813..8401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8575..9432)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9433..9537)
                     /label=AmpR promoter