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YEp24PGK vector (V001491)

Basic Vector Information

Vector Name:
YEp24PGK
Antibiotic Resistance:
Ampicillin
Length:
9637 bp
Type:
Cloning vector
Replication origin:
ori
Host:
Yeast
Source/Author:
Johansson B.
Promoter:
URA3

YEp24PGK vector Vector Map

YEp24PGK9637 bp40080012001600200024002800320036004000440048005200560060006400680072007600800084008800920096002u oriURA3 promoterURA3from Saccharomyces cerevisiae PGK1PGK1 terminatorTcRropbomoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YEp24PGK vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_49537        9637 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector YEp24PGK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Johansson B.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-FEB-2013) Department of Biology, University of Minho, 
            Campus de Gualtar, Braga 4710-051, Portugal
REFERENCE   2  (bases 1 to 9637)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9637)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (01-FEB-2013) Department of Biology, University of Minho, Campus de 
            Gualtar, Braga 4710-051, Portugal"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      348..1690
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        2286..2506
                     /label=URA3 promoter
     CDS             2507..3307
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      3452..4934
                     /label=from Saccharomyces cerevisiae PGK1
                     /note="from Saccharomyces cerevisiae PGK1"
                     /regulatory_class="promoter"
     terminator      5030..5300
                     /label=PGK1 terminator
                     /note="transcription terminator for 3-phosphoglycerate
                     kinase gene"
     CDS             5363..6550
                     /label=TcR
                     /note="tetracycline efflux protein"
     CDS             7194..7382
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    7487..7627
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7813..8401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8575..9432)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9433..9537)
                     /label=AmpR promoter

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