Basic Vector Information
- Vector Name:
- YCp80
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5164 bp
- Type:
- Centromeric vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Baruffini E, Serafini F, Lodi T.
- Promoter:
- TEF
YCp80 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
YCp80 vector Sequence
LOCUS 40924_49452 5164 bp DNA circular SYN 18-DEC-2018
DEFINITION Centromeric vector YCp80, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5164)
AUTHORS Baruffini E, Serafini F, Lodi T.
TITLE Construction and characterization of centromeric, episomal and
GFP-containing vectors for Saccharomyces cerevisiae prototrophic
strains
JOURNAL J. Biotechnol. 143 (4), 247-254 (2009)
PUBMED 19683551
REFERENCE 2 (bases 1 to 5164)
AUTHORS Baruffini E, Serafini F, Lodi T.
TITLE Direct Submission
JOURNAL Submitted (18-MAR-2009) Department of Genetics, Biology of
Microorganisms, Anthropology, Evolution, University of Parma, Viale
Usberti 11/A, Parma 43100, Italy
REFERENCE 3 (bases 1 to 5164)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5164)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J.
Biotechnol."; date: "2009"; volume: "143"; issue: "4"; pages:
"247-254"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(18-MAR-2009) Department of Genetics, Biology of Microorganisms,
Anthropology, Evolution, University of Parma, Viale Usberti 11/A,
Parma 43100, Italy"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5164
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 396..452
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(465..481)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(489..505)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(513..543)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(558..579)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
gene 677..2252
/label=hphMX6
/note="yeast selectable marker conferring hygromycin
resistance"
rep_origin 2409..3229
/label=ARS-CEN6
/note="ARS-CEN6"
rep_origin complement(3345..3933)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4107..4964)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4965..5069)
/label=AmpR promoter
This page is informational only.