YCp80 vector (V001504)

Basic Vector Information

      • Vector Name:
      • YCp80
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5164 bp
      • Type:
      • Centromeric vector
      • Source/Author:
      • Baruffini E, Serafini F, Lodi T.

YCp80 vector Vector Map

YCp805164 bp6001200180024003000360042004800LacZ'lac operatorlac promoterCAP binding siteTEF-promoterTEF-terminatorARS-CEN6oriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YCp80 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5164 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Centromeric vector YCp80, complete sequence.
ACCESSION   FJ834447
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Construction and characterization of centromeric, episomal and 
            GFP-containing vectors for Saccharomyces cerevisiae prototrophic 
            strains
  JOURNAL   J. Biotechnol. 143 (4), 247-254 (2009)
  PUBMED    19683551
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Baruffini E, Serafini F, Lodi T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAR-2009) Department of Genetics, Biology of 
            Microorganisms, Anthropology, Evolution, University of Parma, Viale 
            Usberti 11/A, Parma 43100, Italy
REFERENCE   3  (bases 1 to 5164)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Centromeric vector YCp80"
                     /lab_host="Saccharomyces cerevisiae"
                     /mol_type="other DNA"
                     /db_xref="taxon:670500"
     CDS             complement(146..469)
                     /codon_start=1
                     /gene="lacZ'"
                     /product="LacZ'"
                     /label=lacZ'
                     /note="beta-galactosidase alpha"
                     /protein_id="ACV50010.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     gene            complement(146..469)
                     /gene="lacZ'"
                     /label=lacZ'
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    396..452
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     misc_feature    397..446
                     /note="multiple cloning site; MCS"
     primer_bind     complement(465..481)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    489..505
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(513..543)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    558..579
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     regulatory      631..1020
                     /regulatory_class="promoter"
                     /note="TEF-promoter"
     gene            677..2252
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        677..1020
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1021..2049
                     /codon_start=1
                     /gene="hphR"
                     /product="HphR"
                     /label=hphR
                     /note="aminoglycoside 3'-phosphotransferase (APH)"
                     /protein_id="ACV50011.1"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     gene            1021..2049
                     /gene="hphR"
                     /label=hphR
     regulatory      2050..2281
                     /regulatory_class="terminator"
                     /note="TEF-terminator"
     terminator      2055..2252
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      2409..3229
                     /label=ARS-CEN6
                     /note="ARS-CEN6"
     rep_origin      complement(3345..3933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4104..4964)
                     /codon_start=1
                     /gene="ampR"
                     /product="AmpR"
                     /label=ampR
                     /note="beta-lactamase"
                     /protein_id="ACV50012.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4104..4964)
                     /gene="ampR"
                     /label=ampR
     promoter        complement(4965..5069)
                     /gene="bla"
                     /label=AmpR promoter

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