Basic Vector Information
- Vector Name:
- XOR gate BCD-RFP
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4914 bp
- Type:
- Boolean integrase logic vector
- Replication origin:
- pSC101 ori
- Source/Author:
- Courbet A, Endy D, Renard E, Molina F, Bonnet J.
XOR gate BCD-RFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
XOR gate BCD-RFP vector Sequence
LOCUS 40924_49427 4914 bp DNA circular SYN 18-DEC-2018
DEFINITION Boolean integrase logic vector XOR gate BCD-RFP, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4914)
AUTHORS Courbet A, Endy D, Renard E, Molina F, Bonnet J.
TITLE Detection of pathological biomarkers in human clinical samples via
amplifying genetic switches and logic gates
JOURNAL Sci Transl Med 7 (289), 289RA83 (2015)
PUBMED 26019219
REFERENCE 2 (bases 1 to 4914)
AUTHORS Courbet A, Molina F, Endy D, Bonnet J.
TITLE Direct Submission
JOURNAL Submitted (25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la
Valsiere, Montpellier, Herault 34184, France
REFERENCE 3 (bases 1 to 4914)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4914)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl
Med"; date: "2015"; volume: "7"; issue: "289"; pages: "289RA83"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(25-JUL-2014) SysDiag CNRS UMR3145, CNRS, 1682 Rue de la Valsiere,
Montpellier, Herault 34184, France"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4914
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..22
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
'ATG'"
regulatory 23..69
/label=P7
/note="P7"
/regulatory_class="promoter"
misc_feature 76..128
/label=attB TP901-1
/note="attB TP901-1"
misc_feature 129..178
/label=attB Bxb1
/note="attB Bxb1"
terminator 193..264
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 280..307
/label=T7Te terminator
/note="phage T7 early transcription terminator"
misc_feature 320..372
/label=attP Bxb1
/note="attP Bxb1"
misc_feature 373..422
/label=attP TP901-1
/note="attP TP901-1"
misc_RNA 424..474
/label=sTRSV HHRz
/note="hammerhead ribozyme from the tobacco ringspot virus
satellite RNA (Khvorova et al., 2003)"
misc_feature complement(497..523)
/label=RiboJ
/note="RiboJ"
misc_feature 498..582
/label=BCD2
/note="BCD2"
RBS 568..576
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 580..1275
/label=mKate2
/note="monomeric far-red fluorescent protein (Shcherbo et
al., 2009)"
terminator complement(1301..1344)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1385..1412
/label=T7Te terminator
/note="phage T7 early transcription terminator"
misc_feature 1419..1439
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 1475..1506
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
terminator 1631..1725
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
rep_origin 2217..2439
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
CDS 2487..3434
/label=Rep101
/note="RepA protein needed for replication with the pSC101
origin"
CDS 3726..4583
/label=AmpR
/note="beta-lactamase"
terminator 4597..4656
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
terminator 4716..4745
/label=T3Te terminator
/note="phage T3 early transcription terminator"
terminator complement(4839..4868)
/label=T3Te terminator
/note="phage T3 early transcription terminator"
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