Basic Vector Information
XN21_GW vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
XN21_GW vector Sequence
LOCUS 40924_49412 8919 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector XN21_GW, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8919)
AUTHORS Lalonde S, Sero A, Pratelli R, Pilot G, Chen J, Sardi MI, Parsa SA,
Kim DY, Acharya BR, Stein EV, Hu HC, Villiers F, Takeda K, Yang Y,
Han YS, Schwacke R, Chiang W, Kato N, Loque D, Assmann SM, Kwak JM,
Schroeder JI, Rhee SY, Frommer WB.
TITLE A Membrane Protein/Signaling Protein Interaction Network for
Arabidopsis Version AMPv2
JOURNAL Front Physiol 1, 24 (2010)
PUBMED 21423366
REFERENCE 2 (bases 1 to 8919)
AUTHORS Lalonde S, Frommer WB.
TITLE Direct Submission
JOURNAL Submitted (10-DEC-2010) Plant Biology, Carnegie Institution, 260
Panama St, Stanford, CA 94305, USA
REFERENCE 3 (bases 1 to 8919)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8919)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Front
Physiol 1, 24 (2010)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(10-DEC-2010) Plant Biology, Carnegie Institution, 260 Panama St,
Stanford, CA 94305, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8919
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(2..1344)
/direction=LEFT
/label=2u ori
/note="yeast 2u plasmid origin of replication"
promoter 1603..1883
/label=TRP1 promoter
CDS 1884..2555
/label=TRP1
/note="phosphoribosylanthranilate isomerase, required for
tryptophan biosynthesis"
regulatory 2559..2604
/gene="TRP1"
/regulatory_class="terminator"
rep_origin complement(2661..3116)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 3261..3277
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3302..4008
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
misc_feature 4012..4017
/label=mini-ORF
/note="mini-ORF"
protein_bind 4018..4142
/label=attR1
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(4574..4876)
/label=ccdB
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS complement(5224..5880)
/label=CmR
/note="chloramphenicol acetyltransferase"
promoter complement(5881..5983)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(6161..6285)
/label=attR2
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 6287..6316
/label=linker
/note="linker"
misc_feature 6317..6427
/note="NubG; N-terminal moiety of ubiquitin (ubi4)"
misc_difference 6353..6355
/label=mutation Ile to Gly
/note="mutation Ile to Gly"
CDS 6431..6520
/label=3xHA
/note="three tandem HA epitope tags"
misc_feature 6530..6532
/label=stop codon
/note="stop codon"
terminator 6553..6740
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
primer_bind complement(6768..6784)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6792..6808)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6816..6846)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6861..6882)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7170..7758)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7932..8789)
/label=AmpR
/note="beta-lactamase"
promoter complement(8790..8894)
/label=AmpR promoter
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