VEE(wt).Pac-2A-GFP vector (V001513)

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LOCUS       V001513                14800 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001513
VERSION     V001513
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14800)
  AUTHORS   Kim YG, Baltabekova AZ, Shustov AV.
  TITLE     Poxvirus' interferon inhibitor B18R: recombinant expression,
            refolding and use in mammalian expression system based on viral
            vectors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14800)
  AUTHORS   Kim YG, Baltabekova AZ, Shustov AV.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-MAY-2017) National Center for Biotechnology,
            Korgalzhin hwy 13/5, Astana, Akmola region 010000, Kazakhstan
REFERENCE   3  (bases 1 to 14800)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14800)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-MAY-2017) National Center for Biotechnology, Korgalzhin hwy
            13/5, Astana, Akmola region 010000, Kazakhstan"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14800
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             1536..1544
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7576..8172
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     CDS             8224..8940
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             9063..12827
                     /note="Structural polyprotein from Venezuelan equine
                     encephalitis virus (strain P676). Accession#: P36332"
                     /label="Structural polyprotein"
     promoter        13038..13142
                     /label="AmpR promoter"
     CDS             13143..14000
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      14174..14762
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"