Basic Vector Information
VEE(wt).mCardinal vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
VEE(wt).mCardinal vector Sequence
LOCUS 40924_49347 14173 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector VEE(wt).mCardinal, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14173) AUTHORS Kim YG, Baltabekova AZ, Shustov AV. TITLE Poxvirus' interferon inhibitor B18R: recombinant expression, refolding and use in mammalian expression system based on viral vectors JOURNAL Unpublished REFERENCE 2 (bases 1 to 14173) AUTHORS Kim YG, Baltabekova AZ, Shustov AV. TITLE Direct Submission JOURNAL Submitted (18-MAY-2017) National Center for Biotechnology, Korgalzhin hwy 13/5, Astana, Akmola region 010000, Kazakhstan REFERENCE 3 (bases 1 to 14173) TITLE Direct Submission REFERENCE 4 (bases 1 to 14173) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (18-MAY-2017) National Center for Biotechnology, Korgalzhin hwy 13/5, Astana, Akmola region 010000, Kazakhstan" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..14173 /mol_type="other DNA" /organism="synthetic DNA construct" RBS 1536..1544 /label=Shine-Dalgarno sequence /note="full consensus sequence for ribosome-binding sites upstream of start codons in E. coli; complementary to a region in the 3' end of the 16S rRNA (Chen et al., 1994)" CDS 7582..8313 /label=mCardinal /note="far-red fluorescent protein derived from mNeptune, with further red-shifted spectra" CDS 8436..12200 /note="Structural polyprotein from Venezuelan equine encephalitis virus (strain P676). Accession#: P36332" promoter 12411..12515 /label=AmpR promoter CDS 12516..13373 /label=AmpR /note="beta-lactamase" rep_origin 13547..14135 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 14157..14173 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"
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