Basic Vector Information
pCVD077 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCVD077 vector Sequence
LOCUS 40924_49332 6221 bp DNA circular SYN 18-DEC-2018 DEFINITION Broad host range vector vector pCVD077, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6221) AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. TITLE Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria JOURNAL Nucleic Acids Res. (2014) In press PUBMED 25074377 REFERENCE 2 (bases 1 to 6221) AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW. TITLE Direct Submission JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA REFERENCE 3 (bases 1 to 6221) TITLE Direct Submission REFERENCE 4 (bases 1 to 6221) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res. (2014) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-JUN-2014) Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..6221 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..6 /label=ZraI /note="ZraI" misc_feature 4..24 /note="G5C5; GC-adaptor for seamless assembly, flanking a BHR replicons, neutral sites, or cyanobacterial replicons; synthetic" misc_feature 25..30 /label=XbaI /note="XbaI" misc_recomb 31..1091 /note="A7120NS1-RA; right arm of the homologous recombination site NS1 (alr3858_D748-alr3858_D749) into the chromosome of Anabaena sp. PCC7120 derived from INSD accession NC_003272" misc_feature 31..38 /label=SwaI /note="SwaI" misc_feature 515..520 /label=XbaI /note="XbaI" rep_origin 1128..1716 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(1902..2042) /label=bom /note="basis of mobility region from pBR322" misc_feature 2205..2210 /label=EcoRV /note="EcoRV" CDS complement(2233..3651) /label=SacB /note="secreted levansucrase that renders bacterial growth sensitive to sucrose" regulatory complement(3652..3957) /note="PsacB; derived from Bacillus subtilis INSD accession AAA72307" /regulatory_class="promoter" misc_recomb 3958..4965 /note="A7120NS1-LA; right arm of the homologous recombination site NS1 (alr3858_D748-alr3858_D749) into the chromosome of Anabaena sp. PCC7120 derived from INSD accession NC_003272" misc_feature 4697..4702 /label=MfeI /note="MfeI" misc_feature 4966..4971 /label=NheI /note="NheI" misc_feature 4972..4992 /note="GC; GC-adaptor for seamless assembly, flanking BHR replicons or neutral sites; synthetic" misc_feature 4990..4995 /label=ZraI /note="ZraI" CDS complement(5119..5976) /label=AmpR /note="beta-lactamase" promoter complement(5977..6081) /label=AmpR promoter misc_feature 6107..6112 /label=ZraI /note="ZraI"
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