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vector pCVD076 vector (V001518#)

Basic Vector Information

Basic Vector Information
Vector Name vector pCVD076 Antibiotic Resistance Ampicillin
Length 6207 bp Type Broad host range vector
Source Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

vector pCVD076 vector Vector Map

ZraI ZraI bla AmpR promoter ZraI

vector pCVD076 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6207 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD076, complete sequence.
ACCESSION   KM017876
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6207)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 6207)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 6207)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6207
                     /organism="Broad host range vector vector pCVD076"
                     /mol_type="other DNA"
                     /note="A7120NS2 is a chromosome integration device that 
                     comprises the recombination site NS2 for Anabaena sp 
                     PCC7120, an origin of replication for E coli, a bom site, 
                     for conjugal transfer, from pBR322 and the sacB gene for 
                     selection of double recombinants grown with sucrose A point
                     mutation was identified (G249C) in the ORF of sacB leading 
                     to the following amino acid change: E83D (Ryan Simkovsky) 
                     This device appears however functional in Anabaena sp 
                     PCC7120 and Synechococcus elongatus PCC7942"
                     /db_xref="taxon:1531930"
     misc_feature    1..6
                     /label=ZraI
                     /note="ZraI"
     misc_feature    4..4978
                     /note="CVD-BHR-NS; device for the GC-adaptor assembly: BHR 
                     replicon and neutral sites or right arm of a double 
                     recombination KO"
     misc_feature    4..24
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a 
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    25..30
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     complement(31..1035)
                     /note="A7120NS2-LA; left arm of the homologous 
                     recombination site NS2 (all1997_D43-all1697_D1) into the 
                     chromosome of Anabaena sp. PCC7120 derived from INSD 
                     accession NC_003272"
     rep_origin      1046..1719
                     /note="ori-pBR322; origin of replication of pBR322"
     rep_origin      1072..1660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    1846..1986
                     /label=bom
                     /note="basis of mobility region from pBR322"
     oriT            1892..2005
                     /note="bom; bom site of pBR322, for comjugal transfer; 
                     requires a helper plasmid such as pRL623 (or pRK2013)"
     misc_feature    2149..2154
                     /label=EcoRV
                     /note="EcoRV"
     CDS             complement(2174..3595)
                     /codon_start=1
                     /product="sacB"
                     /label=sacB
                     /note="sacB derived from Bacillus subtilis INSD accession 
                     AAA72307; 1; a point mutation was identified G249C in the 
                     ORF of sacB leading to the following amino acid change: 
                     E83D Ryan Simkovsky; this device appears however functional
                     in Anabaena sp. PCC7120 and Synechococcus elongatus PCC7942
                     Bacillus subtilis"
                     /protein_id="AII99642.1"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     regulatory      complement(3596..3901)
                     /regulatory_class="promoter"
                     /note="PsacB; derived from Bacillus subtilis INSD accession
                     AAA72307"
     misc_recomb     complement(3902..4951)
                     /note="A7120NS2-RA; right arm of the homologous 
                     recombination site NS2 (all1997_D43-all1697_D1) into the 
                     chromosome of Anabaena sp. PCC7120 derived from INSD 
                     accession NC_003272"
     misc_feature    4952..4957
                     /label=NheI
                     /note="NheI"
     misc_feature    4958..4978
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    4976..4981
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(5102..5962)
                     /codon_start=1
                     /product="bla"
                     /label=bla
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pUC19"
                     /protein_id="AII99641.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5963..6067)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(5963..6032)
                     /regulatory_class="promoter"
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"
     misc_feature    6093..6098
                     /label=ZraI
                     /note="ZraI"

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