Cart 2

pCVD048 vector (V001539)

Basic Vector Information

Vector Name:
pCVD048
Antibiotic Resistance:
Ampicillin
Length:
5653 bp
Type:
Broad host range vector
Replication origin:
ori
Source/Author:
Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

pCVD048 vector Vector Map

pCVD0485653 bp60012001800240030003600420048005400ZraIZraIoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pCVD048 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_49222        5653 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD048, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    29..34
                     /label=ZraI
                     /note="ZraI"
     misc_feature    32..3844
                     /note="CVD-CYA; device for the GC-adaptor assembly: 
                     cyanobacterial replicons"
     misc_feature    32..52
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    53..58
                     /label=XbaI
                     /note="XbaI"
     CDS             complement(257..730)
                     /codon_start=1
                     /product="repB"
                     /label=repB
                     /note="repB-pANS; #1 amino acid V to M; similar to INSD 
                     accession AAB21874; Synechococcus elongatus PCC7942"
                     /protein_id="AII99712.1"
                     /translation="MGSCIAPQVRYLHGLKVEELLQRRDWFSVDDPLIQEIVTTATSHR
                     ASAAQILKIQITDKTRASTIVRALLRMIGFKLKARQKRIAGKSTWIYQVVPNEAIAELP
                     LEAIEAHWLENARSSQGLGSCHEIPNRRSKPITAHFRDTASGQVSTTSTETAA"
     CDS             complement(685..3315)
                     /codon_start=1
                     /product="repA"
                     /label=repA
                     /note="repA-pANS; similar to INSD accession AAB21875; 
                     Synechococcus elongatus PCC7942"
                     /protein_id="AII99713.1"
                     /translation="MAFIANHLQRSQIPTRCELIDQLDRLPPNWRFCVVNSRKAPFDPG
                     WQNNPLDHAAVAERIRSDRRVTGIGLLTGPASGGLIAVDFDGPTAHEALPEGLTLTELP
                     PTVAYTSGKPGRHQRLYQVPQERWAAIATQKLHSPDGDLLELRWNKLQSVIVGQHPETG
                     AYRWVEGCAPWEIEVAEAPPELLDAMERQDRQTAPKSYKPIVSAPASDDEVAIARTMLA
                     HVPASYADDYESWVAVGMALQSVSDALLDDWIAWSAQSSKFDGNRKLERKWASFKGSGI
                     TIATLAKLAKEGGYRPPKLTRQEQREYQREVANEWQRLTRSPNRLHTGGYINGDYIPQP
                     EQHRLVCLNAAMGTGKTEAIAGHLQPLIGTGIPVVLITHRRTLGAALGKRLGVPWAEEC
                     VPGSDLRMIGLGGCLDSMHPASKLRFSAHGWQRAIVVIDEVELWAEHLLSGKTEIAKHR
                     PEVMAEIAGLLAGGRQVIAADAMLSDVSIALLEALTGSTAYLVSSTVQPFSGAPVYQVG
                     SDRELMAMAVEMVQAGKRLAVFCDRQKLSQSRPSLYSAATIAQQLSEACGQPVPVIDSE
                     ANKRGALETLKLNPQMFINCCPHLVFTSVIDAGLSITQGVGDRPFDAVLIFAGAGHIAP
                     GAIAQIAGRVRSNVPRFITCPDTSNLMRAGNGSIDPTEVADGLKRHQQVILAQLSQAAI
                     KIGEPVASQAYLTAWAELAARRNRDGLTYAATVEQLLRLNGFTWQDWDGAIDPFLQEVV
                     DELQRETAEALADASAEAILSSPDLTPKEAEAIASADFANAEQLAALARHRCQQRWGDC
                     TRELLAADDDGLYAPLRLLFWLSLGKDAVPLAMPPSSTNGAIARCSDPILWAAVSRPKC
                     GTCMG"
     misc_feature    3818..3823
                     /label=SacI
                     /note="SacI"
     misc_feature    3824..3844
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons; synthetic"
     misc_feature    3842..3847
                     /label=ZraI
                     /note="ZraI"
     rep_origin      complement(3929..4517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4691..5548)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5549..5653)
                     /label=AmpR promoter

This page is informational only.