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vector pCVD048 vector (V001539#)

Basic Vector Information

Basic Vector Information
Vector Name vector pCVD048 Antibiotic Resistance Ampicillin
Length 5653 bp Type Broad host range vector
Source Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

vector pCVD048 vector Vector Map

ZraI ZraI ori-pUC19 bla AmpR promoter

vector pCVD048 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5653 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD048, complete sequence.
ACCESSION   KM017899
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Broad host range vector vector pCVD048"
                     /mol_type="other DNA"
                     /note="pANS is a replication device carrying a fragment of 
                     pANS (the small plasmid of Synechococcus elongatus PCC7942)
                     including repA and repB for replication of the plasmid in S
                     elongatus"
                     /db_xref="taxon:1531906"
     misc_feature    29..34
                     /label=ZraI
                     /note="ZraI"
     misc_feature    32..3844
                     /note="CVD-CYA; device for the GC-adaptor assembly: 
                     cyanobacterial replicons"
     misc_feature    32..52
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a 
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    53..58
                     /label=XbaI
                     /note="XbaI"
     CDS             complement(257..730)
                     /codon_start=1
                     /product="repB"
                     /label=repB
                     /note="repB-pANS; #1 amino acid V to M; similar to INSD 
                     accession AAB21874; Synechococcus elongatus PCC7942"
                     /protein_id="AII99712.1"
                     /translation="MGSCIAPQVRYLHGLKVEELLQRRDWFSVDDPLIQEIVTTATSHR
                     ASAAQILKIQITDKTRASTIVRALLRMIGFKLKARQKRIAGKSTWIYQVVPNEAIAELP
                     LEAIEAHWLENARSSQGLGSCHEIPNRRSKPITAHFRDTASGQVSTTSTETAA"
     CDS             complement(685..3315)
                     /codon_start=1
                     /product="repA"
                     /label=repA
                     /note="repA-pANS; similar to INSD accession AAB21875; 
                     Synechococcus elongatus PCC7942"
                     /protein_id="AII99713.1"
                     /translation="MAFIANHLQRSQIPTRCELIDQLDRLPPNWRFCVVNSRKAPFDPG
                     WQNNPLDHAAVAERIRSDRRVTGIGLLTGPASGGLIAVDFDGPTAHEALPEGLTLTELP
                     PTVAYTSGKPGRHQRLYQVPQERWAAIATQKLHSPDGDLLELRWNKLQSVIVGQHPETG
                     AYRWVEGCAPWEIEVAEAPPELLDAMERQDRQTAPKSYKPIVSAPASDDEVAIARTMLA
                     HVPASYADDYESWVAVGMALQSVSDALLDDWIAWSAQSSKFDGNRKLERKWASFKGSGI
                     TIATLAKLAKEGGYRPPKLTRQEQREYQREVANEWQRLTRSPNRLHTGGYINGDYIPQP
                     EQHRLVCLNAAMGTGKTEAIAGHLQPLIGTGIPVVLITHRRTLGAALGKRLGVPWAEEC
                     VPGSDLRMIGLGGCLDSMHPASKLRFSAHGWQRAIVVIDEVELWAEHLLSGKTEIAKHR
                     PEVMAEIAGLLAGGRQVIAADAMLSDVSIALLEALTGSTAYLVSSTVQPFSGAPVYQVG
                     SDRELMAMAVEMVQAGKRLAVFCDRQKLSQSRPSLYSAATIAQQLSEACGQPVPVIDSE
                     ANKRGALETLKLNPQMFINCCPHLVFTSVIDAGLSITQGVGDRPFDAVLIFAGAGHIAP
                     GAIAQIAGRVRSNVPRFITCPDTSNLMRAGNGSIDPTEVADGLKRHQQVILAQLSQAAI
                     KIGEPVASQAYLTAWAELAARRNRDGLTYAATVEQLLRLNGFTWQDWDGAIDPFLQEVV
                     DELQRETAEALADASAEAILSSPDLTPKEAEAIASADFANAEQLAALARHRCQQRWGDC
                     TRELLAADDDGLYAPLRLLFWLSLGKDAVPLAMPPSSTNGAIARCSDPILWAAVSRPKC
                     GTCMG"
     misc_feature    3818..3823
                     /label=SacI
                     /note="SacI"
     misc_feature    3824..3844
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons; synthetic"
     misc_feature    3842..3847
                     /label=ZraI
                     /note="ZraI"
     rep_origin      complement(3870..4543)
                     /direction=LEFT
                     /note="ori-pUC19; origin of replication of pUC19"
     rep_origin      complement(3929..4517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4688..5548)
                     /codon_start=1
                     /product="bla"
                     /label=bla
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pUC19"
                     /protein_id="AII99711.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5549..5653)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(5549..5618)
                     /regulatory_class="promoter"
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"

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