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vector pCVD046 vector (V001541#)

Basic Vector Information

Basic Vector Information
Vector Name vector pCVD046 Antibiotic Resistance Ampicillin
Length 7140 bp Type Broad host range vector
Source Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

vector pCVD046 vector Vector Map

bla AmpR promoter ZraI ZraI ZraI

vector pCVD046 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                7140 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD046, complete sequence.
ACCESSION   KM017897
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7140)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 7140)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 7140)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7140
                     /organism="Broad host range vector vector pCVD046"
                     /mol_type="other DNA"
                     /note="RSF1010 is a replication device carrying a broad 
                     host range origin of replication, an origin of transfer, 
                     rep and mob genes derived from RSF1010"
                     /db_xref="taxon:1531904"
     CDS             complement(121..981)
                     /codon_start=1
                     /product="bla"
                     /label=bla
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pUC19"
                     /protein_id="AII99693.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(982..1086)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(982..1051)
                     /regulatory_class="promoter"
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"
     misc_feature    1112..1117
                     /label=ZraI
                     /note="ZraI"
     misc_feature    1227..1232
                     /label=ZraI
                     /note="ZraI"
     misc_feature    1230..7137
                     /note="CVD-BHR-NS; device for the GC-adaptor assembly: BHR 
                     replicon and neutral sites or right arm of a double 
                     recombination KO"
     misc_feature    1230..1250
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a 
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    1251..1256
                     /label=XbaI
                     /note="XbaI"
     regulatory      1288..1314
                     /regulatory_class="terminator"
                     /note="trpA T; trpA terminator from Lorist6 INSD accession 
                     X98450, one nt shorter than reference; Lorist6"
     CDS             complement(1647..2498)
                     /codon_start=1
                     /product="repC"
                     /label=repC
                     /note="repC-RSF1010; replication gene; derived from 
                     pRL1383a in INSD accession AF403426, a derivative of 
                     RSF1010 INSD accession M28829"
                     /protein_id="AII99700.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRKRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2485..3324)
                     /codon_start=1
                     /product="repA"
                     /label=repA
                     /note="repA-RSF1010; replication gene; derived from 
                     pRL1383a in INSD accession AF403426, a derivative of 
                     RSF1010 INSD accession M28829"
                     /protein_id="AII99699.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(3354..3560)
                     /codon_start=1
                     /product="repF"
                     /label=repF
                     /note="repF-RSF1010; replication gene; derived from 
                     pRL1383a in INSD accession AF403426, a derivative of 
                     RSF1010 INSD accession M28829"
                     /protein_id="AII99697.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             complement(3562..3774)
                     /codon_start=1
                     /product="repE"
                     /label=repE
                     /note="repE-RSF1010; replication gene; derived from 
                     pRL1383a in INSD accession AF403426, a derivative of 
                     RSF1010 INSD accession M28829"
                     /protein_id="AII99698.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     regulatory      complement(3804..3831)
                     /regulatory_class="promoter"
                     /note="P4; derived from pRL1383a in INSD accession 
                     AF403426, a derivative of RSF1010 INSD accession M28829; 
                     the P4 promoter is under the auto-regulated control of the 
                     F repressor, whose gene is located in the same operon, 
                     providing transcription of E-F-repA-repC-operon"
     CDS             complement(3835..5964)
                     /codon_start=1
                     /product="mobA"
                     /label=mobA
                     /note="mobilization gene; derived from pRL1383a in INSD 
                     accession AF403426, a derivative of RSF1010 INSD accession 
                     M28829"
                     /protein_id="AII99696.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDQAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDAPTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGHDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
     CDS             complement(3835..4806)
                     /codon_start=1
                     /product="repB"
                     /label=repB
                     /note="repA-RSF1010; replication gene; derived from 
                     pRL1383a in INSD accession AF403426, a derivative of 
                     RSF1010 INSD accession M28829"
                     /protein_id="AII99701.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDAPTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGHDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     misc_feature    4735..4740
                     /label=EcoRV
                     /note="EcoRV"
     CDS             complement(4803..5216)
                     /codon_start=1
                     /product="mobB"
                     /label=mobB
                     /note="mobilization gene; derived from pRL1383a in INSD 
                     accession AF403426, a derivative of RSF1010 INSD accession 
                     M28829"
                     /protein_id="AII99695.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTKQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     misc_feature    5662..5667
                     /label=AgeI
                     /note="AgeI"
     oriT            6045..6132
                     /note="oriT; origin of transfer from conjugation; from 
                     pRL1383a in INSD accession AF403426, a derivative of 
                     RSF1010 INSD accession M288298"
     misc_feature    6063..6068
                     /label=AgeI
                     /note="AgeI"
     regulatory      6099..6130
                     /regulatory_class="promoter"
                     /note="P2; derived from pRL1383a in INSD accession 
                     AF403426, a derivative of RSF1010 INSD accession M28829; 
                     the P2 promoter is controlled by MobC and MobA provide 
                     transcription of mobC"
     regulatory      complement(6122..6152)
                     /regulatory_class="promoter"
                     /note="P3; derived from pRL1383a in INSD accession 
                     AF403426, a derivative of RSF1010 INSD accession M28829; 
                     the P3 is controlled by MobC and MobA provide transcription
                     of mobA/repB, mobB, and, probably, E-F-repA-repC-operon"
     regulatory      complement(6141..6172)
                     /regulatory_class="promoter"
                     /note="P1; derived from pRL1383a in INSD accession 
                     AF403426, a derivative of RSF1010 INSD accession M28829; 
                     the P1 promoter is controlled by MobC and MobA provide 
                     transcription of mobA/repB, mobB, and, probably, 
                     E-F-repA-repC-operon"
     CDS             6163..6447
                     /codon_start=1
                     /product="mobC"
                     /label=mobC
                     /note="mobilization gene; derived from pRL1383a in INSD 
                     accession AF403426, a derivative of RSF1010 INSD accession 
                     M28829"
                     /protein_id="AII99694.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRERAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     rep_origin      complement(6380..6879)
                     /direction=LEFT
                     /note="oriV; origin of replication; derived from pRL1383a 
                     in INSD accession AF403426, a derivative of RSF1010 INSD 
                     accession M28829"
     rep_origin      6474..6868
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     terminator      7035..7078
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     regulatory      complement(7044..7072)
                     /regulatory_class="terminator"
                     /note="TrrnC; rrnC terminator from Lorist6 INSD accession 
                     X98450; Lorist6"
     misc_feature    7111..7116
                     /label=NheI
                     /note="NheI"
     misc_feature    7117..7137
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    7135..7140
                     /label=ZraI
                     /note="ZraI"

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