Basic Vector Information
pCVD035 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCVD035 vector Sequence
LOCUS 40924_49187 3493 bp DNA circular SYN 18-DEC-2018
DEFINITION Broad host range vector vector pCVD035, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3493)
AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B,
Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
TITLE Broad-host-range vector system for synthetic biology and
biotechnology in cyanobacteria
JOURNAL Nucleic Acids Res. (2014) In press
PUBMED 25074377
REFERENCE 2 (bases 1 to 3493)
AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B,
Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
TITLE Direct Submission
JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University
of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE 3 (bases 1 to 3493)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3493)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res. (2014) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-JUN-2014) Division of Biological Sciences, University of
California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3493
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 293..309
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 320..338
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 389..394
/label=EcoRV
/note="EcoRV"
misc_feature 392..412
/note="C2G; GC-adaptor for seamless assembly, flanking
antibiotic resistance markers, functional devices or custom
sequences; synthetic"
misc_feature 413..418
/label=AgeI
/note="AgeI"
regulatory 428..618
/note="PpsbAI_S7942; upstream region of psbA1 up to 25nt
upstream of the start codon in Synechococcus elongatus
PCC7942"
/regulatory_class="promoter"
regulatory 625..665
/note="RBS_LT; RBS and flanking sequences found in Life
Tech pENTR-SD/D Topo, start downstream of the att site and
includes the T7 gene enhancer, RBS, TOPO binding site and
directional TOPO overhang; Life Technologies; synthetic"
/regulatory_class="ribosome_binding_site"
RBS 636..656
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 666..1382
/label=EYFP
/note="enhanced YFP"
protein_bind complement(1402..1426)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
terminator 1436..1463
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
misc_feature 1472..1477
/label=XbaI
/note="XbaI"
misc_feature 1478..1498
/note="G5C5; GC-adaptor for seamless assembly, flanking
functional devices, custom sequences or cyanobacterial
replicons; synthetic"
misc_feature 1496..1501
/label=EcoRV
/note="EcoRV"
rep_origin complement(1746..2334)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2508..3365)
/label=AmpR
/note="beta-lactamase"
promoter complement(3366..3470)
/label=AmpR promoter
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