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vector pCVD031 vector (V001550#)

Basic Vector Information

Basic Vector Information
Vector Name vector pCVD031 Length 3331 bp
Type Broad host range vector Source Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

vector pCVD031 vector Vector Map

M13 fwd T7 promoter EcoRV EcoRV colE1_pMA-RQ bla_pMA-RQ AmpR promoter

vector pCVD031 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3331 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD031, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 3331)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Broad host range vector vector pCVD031"
                     /mol_type="other DNA"
                     /note="PconII-LTRBS-yemGFP is a functional device where 
                     yemGFP is driven by a PconII promoter; RBS and flanking 
                     sequences from the pENTR/SD/D-Topo vector"
     primer_bind     293..309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
     promoter        320..338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    389..394
     misc_feature    392..1336
                     /note="CVD-cassette; device for the GC-adaptor assembly: 
                     expression/reporter cassette, promoter-reporter module, 
                     custom sequence"
     misc_feature    392..412
                     /note="C2G; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers, functional devices or custom
                     sequences; synthetic"
     misc_feature    413..418
     regulatory      425..459
                     /note="PconII; -35 to +1 region derived from conII 
                     synthetic promoter; synthetic"
     promoter        425..453
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     regulatory      466..506
                     /note="RBS_LT; RBS and flanking sequences found in Life 
                     Tech pENTR-SD/D Topo, start downstream of the att site and 
                     includes the T7 gene enhancer, RBS, TOPO binding site and 
                     directional TOPO overhang; Life Technologies; synthetic"
     RBS             477..497
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             507..1223
                     /note="expression plasmid containing yemGFP, a GFP allele 
                     with F64L, S65T, and A206K mutations and that has been 
                     codon-optimized for expression in yeast; the first two 
                     mutations correspond to GFPmut1, and the third mutation 
                     interferes with GFP dimerization; Aequorea victoria"
     misc_feature    720..725
     misc_feature    1066..1071
     protein_bind    complement(1240..1264)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1274..1302
                     /note="rrnB T2; rrnB T2; rrnB T2 terminator partial 
     terminator      1274..1301
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
     misc_feature    1310..1315
     misc_feature    1316..1336
                     /note="G5C5; GC-adaptor for seamless assembly, flanking 
                     functional devices, custom sequences or cyanobacterial 
                     replicons; synthetic"
     misc_feature    1334..1339
     rep_origin      complement(1525..2198)
                     /note="colE1_pMA-RQ; origin of replication of colE1 found 
                     inderived from pMA-RQ GeneART vector"
     rep_origin      complement(1584..2172)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
     CDS             complement(2343..3203)
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pMA-RQ GeneART vector, has one point mutation 
                     compared to pUC19"
     promoter        complement(3204..3308)
                     /label=AmpR promoter
     regulatory      complement(3204..3273)
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"

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