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vector pCVD029 vector (V001552#)

Basic Vector Information

Basic Vector Information
Vector Name vector pCVD029 Antibiotic Resistance Ampicillin
Length 4487 bp Type Broad host range vector
Source Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

vector pCVD029 vector Vector Map

lacZa-part2 EcoRV EcoRV lacZa-part1 PlacZa ori-pUC19 bla AmpR promoter ZraI

vector pCVD029 vector Sequence

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Download GeneBank File(.gb)

LOCUS       Exported                4487 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD029, complete sequence.
ACCESSION   KM017917
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4487)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Broad host range vector vector pCVD029"
                     /mol_type="other DNA"
                     /note="pBAV1K is a replication device for E coli, possibly 
                     broad host range, that can be assembled with a 
                     cyanobacterial replicon"
                     /db_xref="taxon:1531887"
     misc_feature    complement(146..404)
                     /note="lacZa-part2; lacZalpha fragment upstream of EcoICRI"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    405..410
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    408..2202
                     /note="CVD-ECcya; device for the GC-adaptor assembly: 
                     Escherichia coli origin to be assembled with a 
                     cyanobacterial replicon"
     misc_feature    408..428
                     /note="C3G3; GC-adaptor for seamless assembly, flanking 
                     cyanobacterial replicons or Escherichia coli origins to be 
                     assembled with a cyanobacterial replicon; synthetic"
     misc_feature    429..434
                     /label=MfeI
                     /note="MfeI"
     misc_feature    434..439
                     /label=SacI
                     /note="SacI"
     regulatory      complement(435..548)
                     /regulatory_class="terminator"
                     /note="T0; derived from pBAV1K, Escherichia coli rrfB 
                     terminator"
     terminator      445..539
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(741..752)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      2083..2169
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     regulatory      2098..2175
                     /regulatory_class="terminator"
                     /note="T1; derived from pBAV1K, Escherichia coli rrfB 
                     terminator"
     misc_feature    2176..2181
                     /label=NheI
                     /note="NheI"
     misc_feature    2182..2202
                     /note="GC; GC-adaptor for seamless assembly, flanking 
                     antibiotic resistance markers or Escherichia coli origins 
                     to be assembled with a cyanobacterial replicon; synthetic"
     misc_feature    2200..2205
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    complement(2206..2270)
                     /note="lacZa-part1; lacZalpha fragment upstream of EcoICRI"
     misc_feature    2224..2229
                     /label=XbaI
                     /note="XbaI"
     primer_bind     complement(2266..2282)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      complement(2271..2393)
                     /regulatory_class="promoter"
                     /note="PlacZa; lacI-lacZ intergenic region derived from 
                     Escherichia coli including the promoter and RBS"
     regulatory      complement(2288..2308)
                     /regulatory_class="other"
                     /note="lacO; LacO; lac operator (lacO) from Invitrogen 
                     pTrcHis2"
     protein_bind    2290..2306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2314..2344)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2359..2380
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(2609..3282)
                     /direction=LEFT
                     /note="ori-pUC19; origin of replication of pUC19"
     rep_origin      complement(2668..3256)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3427..4287)
                     /codon_start=1
                     /product="bla"
                     /label=bla
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pUC19"
                     /protein_id="AII99745.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4288..4392)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(4288..4357)
                     /regulatory_class="promoter"
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"
     misc_feature    4418..4423
                     /label=ZraI
                     /note="ZraI"

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