pCVD025 vector (V001556)

Basic Vector Information

      • Vector Name:
      • pCVD025
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4141 bp
      • Type:
      • Broad host range vector
      • Source/Author:
      • Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

pCVD025 vector Vector Map

pCVD0254141 bp60012001800240030003600ZraIXbaIS7942NS3-RA; right arm of the homologous recombination site NS3 into the chromosome of Synechococcus elongatus PCC7942; from pHN1_lacUV5, INSD accession NC_007604, HM026758orioriTS7942NS3-LA; left arm of the homologous recombination site NS3 into the chromosome of Synechococcus elongatus PCC7942; from pHN1_lacUV5, INSD accession NC_007604, HM026758NheIGC; GC-adaptor for seamless assembly, flanking BHR replicons or neutral sites; syntheticAmpRAmpR promoterZraI

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pCVD025 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_49137        4141 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD025, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4141)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4141)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..877
                     /note="S7942NS3-RA; right arm of the homologous
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     rep_origin      914..1502
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            complement(1743..1852)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     misc_recomb     2039..2930
                     /note="S7942NS3-LA; left arm of the homologous
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     misc_feature    2931..2936
                     /label=NheI
                     /note="NheI"
     misc_feature    2937..2957
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2955..2960
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(3084..3941)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3942..4046)
                     /label=AmpR promoter
     misc_feature    4072..4077
                     /label=ZraI
                     /note="ZraI"

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