Cart 2

vector pCVD024 vector (V001557#)

Basic Vector Information

Basic Vector Information
Vector Name vector pCVD024 Antibiotic Resistance Ampicillin
Length 4012 bp Type Broad host range vector
Source Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

vector pCVD024 vector Vector Map

ZraI ZraI bla AmpR promoter ZraI

vector pCVD024 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4012 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD024, complete sequence.
ACCESSION   KM017925
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 4012)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Broad host range vector vector pCVD024"
                     /mol_type="other DNA"
                     /note="S7942NS3 is a chromosome integration device that 
                     comprises the recombination site NS3 for Synechococcus 
                     elongatus PCC7942, an origin of replication for E coli and 
                     a bom site for conjugal transfer from pBR322"
                     /db_xref="taxon:1531882"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..2828
                     /note="CVD-BHR-NS; device for the GC-adaptor assembly: BHR 
                     replicon and neutral sites or right arm of a double 
                     recombination KO"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a 
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..877
                     /note="S7942NS3-RA; right arm of the homologous 
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     rep_origin      888..1561
                     /note="ori-pBR322; origin of replication of pBR322"
     rep_origin      914..1502
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    1688..1828
                     /label=bom
                     /note="basis of mobility region from pBR322"
     oriT            1734..1847
                     /note="bom; bom site of pBR322, for comjugal transfer; 
                     requires a helper plasmid such as pRL623 (or pRK2013)"
     misc_recomb     1910..2801
                     /note="S7942NS3-LA; left arm of the homologous 
                     recombination site NS3 into the chromosome of Synechococcus
                     elongatus PCC7942; from pHN1_lacUV5, INSD accession 
                     NC_007604, HM026758"
     misc_feature    2802..2807
                     /label=NheI
                     /note="NheI"
     misc_feature    2808..2828
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    2826..2831
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(2952..3812)
                     /codon_start=1
                     /product="bla"
                     /label=bla
                     /note="confers ampicillin resistance; beta-lactamase; 
                     derived from pUC19"
                     /protein_id="AII99757.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3813..3917)
                     /gene="bla"
                     /label=AmpR promoter
     regulatory      complement(3813..3882)
                     /regulatory_class="promoter"
                     /note="Pbla; upstream region of the bla ORF including 
                     promoter and RBS; derived from pUC19"
     misc_feature    3943..3948
                     /label=ZraI
                     /note="ZraI"

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