Basic Vector Information
- Vector Name:
- pCVD023
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5124 bp
- Type:
- Broad host range vector
- Replication origin:
- ori
- Source/Author:
- Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
- Promoter:
- tet
pCVD023 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCVD023 vector Sequence
LOCUS 40924_49127 5124 bp DNA circular SYN 18-DEC-2018
DEFINITION Broad host range vector vector pCVD023, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5124)
AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B,
Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
TITLE Broad-host-range vector system for synthetic biology and
biotechnology in cyanobacteria
JOURNAL Nucleic Acids Res. (2014) In press
PUBMED 25074377
REFERENCE 2 (bases 1 to 5124)
AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B,
Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
TITLE Direct Submission
JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University
of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE 3 (bases 1 to 5124)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5124)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res. (2014) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-JUN-2014) Division of Biological Sciences, University of
California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5124
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 46..51
/label=ZraI
/note="ZraI"
misc_feature 49..69
/note="G5C5; GC-adaptor for seamless assembly, flanking a
BHR replicons, neutral sites, or cyanobacterial replicons;
synthetic"
misc_feature 70..75
/label=XbaI
/note="XbaI"
misc_recomb 76..536
/note="S7942NS2-RA; right arm of the homologous
recombination site NS2 into the chromosome of Synechococcus
elongatus PCC7942 derived from INSD accession NC_007604,
U44761"
misc_feature 445..450
/label=SacI
/note="SacI"
rep_origin 573..1161
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(1347..1487)
/label=bom
/note="basis of mobility region from pBR322"
misc_feature complement(1567..1656)
/note="tet-3UTR; 3' UTR of the tetracyclin resistance ORF;
from pACYC184 INSD accession X06403"
CDS complement(1660..2847)
/label=TcR
/note="tetracycline efflux protein"
promoter complement(2895..2923)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
misc_recomb 2972..3913
/note="S7942NS2-LA; left arm of the homologous
recombination site NS2 into the chromosome of Synechococcus
elongatus PCC7942 derived from INSD accession NC_007604,
U44761"
misc_feature 3914..3919
/label=NheI
/note="NheI"
misc_feature 3920..3940
/note="GC; GC-adaptor for seamless assembly, flanking BHR
replicons or neutral sites; synthetic"
misc_feature 3938..3943
/label=ZraI
/note="ZraI"
CDS complement(4067..4924)
/label=AmpR
/note="beta-lactamase"
promoter complement(4925..5029)
/label=AmpR promoter
misc_feature 5055..5060
/label=ZraI
/note="ZraI"
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