Basic Vector Information
pCVD022 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCVD022 vector Sequence
LOCUS 40924_49122 3850 bp DNA circular SYN 18-DEC-2018
DEFINITION Broad host range vector vector pCVD022, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3850)
AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B,
Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
TITLE Broad-host-range vector system for synthetic biology and
biotechnology in cyanobacteria
JOURNAL Nucleic Acids Res. (2014) In press
PUBMED 25074377
REFERENCE 2 (bases 1 to 3850)
AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B,
Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
TITLE Direct Submission
JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University
of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE 3 (bases 1 to 3850)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3850)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res. (2014) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-JUN-2014) Division of Biological Sciences, University of
California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3850
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 46..51
/label=ZraI
/note="ZraI"
misc_feature 49..69
/note="G5C5; GC-adaptor for seamless assembly, flanking a
BHR replicons, neutral sites, or cyanobacterial replicons;
synthetic"
misc_feature 70..75
/label=XbaI
/note="XbaI"
misc_recomb 76..536
/note="S7942NS2-RA; right arm of the homologous
recombination site NS2 into the chromosome of Synechococcus
elongatus PCC7942 derived from INSD accession NC_007604,
U44761"
misc_feature 445..450
/label=SacI
/note="SacI"
rep_origin 573..1161
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
oriT complement(1402..1511)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
misc_recomb 1698..2639
/note="S7942NS2-LA; left arm of the homologous
recombination site NS2 into the chromosome of Synechococcus
elongatus PCC7942 derived from INSD accession NC_007604,
U44761"
misc_feature 2640..2645
/label=NheI
/note="NheI"
misc_feature 2646..2666
/note="GC; GC-adaptor for seamless assembly, flanking BHR
replicons or neutral sites; synthetic"
misc_feature 2664..2669
/label=ZraI
/note="ZraI"
CDS complement(2793..3650)
/label=AmpR
/note="beta-lactamase"
promoter complement(3651..3755)
/label=AmpR promoter
misc_feature 3781..3786
/label=ZraI
/note="ZraI"
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