Basic Vector Information
pCVD020 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCVD020 vector Sequence
LOCUS 40924_49112 5372 bp DNA circular SYN 18-DEC-2018 DEFINITION Broad host range vector vector pCVD020, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5372) AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. TITLE Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria JOURNAL Nucleic Acids Res. (2014) In press PUBMED 25074377 REFERENCE 2 (bases 1 to 5372) AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW. TITLE Direct Submission JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA REFERENCE 3 (bases 1 to 5372) TITLE Direct Submission REFERENCE 4 (bases 1 to 5372) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res. (2014) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-JUN-2014) Division of Biological Sciences, University of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5372 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 46..51 /label=ZraI /note="ZraI" misc_feature 49..69 /note="G5C5; GC-adaptor for seamless assembly, flanking a BHR replicons, neutral sites, or cyanobacterial replicons; synthetic" misc_feature 70..75 /label=XbaI /note="XbaI" misc_recomb 76..855 /note="S7942NS1-RA; right arm of the homologous recombination site NS1 into the chromosome of Synechococcus elongatus PCC7942 derived from INSD accession NC_007604, U30252" rep_origin 892..1480 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(1666..1806) /label=bom /note="basis of mobility region from pBR322" promoter 1930..1958 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" CDS 2006..3193 /label=TcR /note="tetracycline efflux protein" misc_feature 3197..3286 /note="tet-3UTR; 3' UTR of the tetracyclin resistance ORF; from pACYC184 INSD accession X06403" CDS complement(3428..3874) /gene="ndk" /label=ndk /note="Nucleoside diphosphate kinase from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805). Accession#: P50590" misc_feature 3880..3885 /label=XbaI /note="XbaI" misc_feature 4067..4072 /label=NheI /note="NheI" misc_feature 4127..4132 /label=EcoRV /note="EcoRV" misc_feature 4162..4167 /label=NheI /note="NheI" misc_feature 4168..4188 /note="GC; GC-adaptor for seamless assembly, flanking BHR replicons or neutral sites; synthetic" misc_feature 4186..4191 /label=ZraI /note="ZraI" CDS complement(4315..5172) /label=AmpR /note="beta-lactamase" promoter complement(5173..5277) /label=AmpR promoter misc_feature 5303..5308 /label=ZraI /note="ZraI"
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