pCVD020 vector (V001561)

Basic Vector Information

      • Vector Name:
      • pCVD020
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5372 bp
      • Type:
      • Broad host range vector
      • Source/Author:
      • Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.

pCVD020 vector Vector Map

pCVD0205372 bp6001200180024003000360042004800ZraIXbaIS7942NS1-RA; right arm of the homologous recombination site NS1 into the chromosome of Synechococcus elongatus PCC7942 derived from INSD accession NC_007604, U30252oribomtet promoterTcRtet-3UTR; 3' UTR of the tetracyclin resistance ORF; from pACYC184 INSD accession X06403ndkXbaINheIEcoRVNheIGC; GC-adaptor for seamless assembly, flanking BHR replicons or neutral sites; syntheticAmpRAmpR promoterZraI

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pCVD020 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_49112        5372 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range vector vector pCVD020, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
  PUBMED    25074377
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B, 
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University 
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5372)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5372)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. (2014) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-JUN-2014) Division of Biological Sciences, University of 
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label=ZraI
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons; 
                     synthetic"
     misc_feature    70..75
                     /label=XbaI
                     /note="XbaI"
     misc_recomb     76..855
                     /note="S7942NS1-RA; right arm of the homologous
                     recombination site NS1 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604, 
                     U30252"
     rep_origin      892..1480
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(1666..1806)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        1930..1958
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2006..3193
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_feature    3197..3286
                     /note="tet-3UTR; 3' UTR of the tetracyclin resistance ORF;
                     from pACYC184 INSD accession X06403"
     CDS             complement(3428..3874)
                     /gene="ndk"
                     /label=ndk
                     /note="Nucleoside diphosphate kinase from Synechococcus 
                     elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805). 
                     Accession#: P50590"
     misc_feature    3880..3885
                     /label=XbaI
                     /note="XbaI"
     misc_feature    4067..4072
                     /label=NheI
                     /note="NheI"
     misc_feature    4127..4132
                     /label=EcoRV
                     /note="EcoRV"
     misc_feature    4162..4167
                     /label=NheI
                     /note="NheI"
     misc_feature    4168..4188
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR 
                     replicons or neutral sites; synthetic"
     misc_feature    4186..4191
                     /label=ZraI
                     /note="ZraI"
     CDS             complement(4315..5172)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5173..5277)
                     /label=AmpR promoter
     misc_feature    5303..5308
                     /label=ZraI
                     /note="ZraI"

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