pCVD020 vector (V001561)

LOCUS       V001561                 5372 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001561
VERSION     V001561
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B,
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Broad-host-range vector system for synthetic biology and
            biotechnology in cyanobacteria
  JOURNAL   Nucleic Acids Res. (2014) In press
   PUBMED   25074377
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B,
            Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUN-2014) Division of Biological Sciences, University
            of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE   3  (bases 1 to 5372)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5372)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
            Acids Res. (2014) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (17-JUN-2014) Division of Biological Sciences, University of
            California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..51
                     /label="ZraI"
                     /note="ZraI"
     misc_feature    49..69
                     /note="G5C5; GC-adaptor for seamless assembly, flanking a
                     BHR replicons, neutral sites, or cyanobacterial replicons;
                     synthetic"
     misc_feature    70..75
                     /label="XbaI"
                     /note="XbaI"
     misc_recomb     76..855
                     /note="S7942NS1-RA; right arm of the homologous
                     recombination site NS1 into the chromosome of Synechococcus
                     elongatus PCC7942 derived from INSD accession NC_007604,
                     U30252"
     rep_origin      892..1480
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(1666..1806)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     promoter        1930..1958
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2006..3193
                     /label="TcR"
                     /note="tetracycline efflux protein"
     misc_feature    3197..3286
                     /note="tet-3UTR; 3' UTR of the tetracyclin resistance ORF;
                     from pACYC184 INSD accession X06403"
     CDS             complement(3428..3874)
                     /gene="ndk"
                     /label="Nucleoside diphosphate kinase"
                     /note="Nucleoside diphosphate kinase from Synechococcus
                     elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805).
                     Accession#: P50590"
     misc_feature    3880..3885
                     /label="XbaI"
                     /note="XbaI"
     misc_feature    4067..4072
                     /label="NheI"
                     /note="NheI"
     misc_feature    4127..4132
                     /label="EcoRV"
                     /note="EcoRV"
     misc_feature    4162..4167
                     /label="NheI"
                     /note="NheI"
     misc_feature    4168..4188
                     /note="GC; GC-adaptor for seamless assembly, flanking BHR
                     replicons or neutral sites; synthetic"
     misc_feature    4186..4191
                     /label="ZraI"
                     /note="ZraI"
     CDS             complement(4315..5172)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5173..5277)
                     /label="AmpR promoter"
     misc_feature    5303..5308
                     /label="ZraI"
                     /note="ZraI"