Basic Vector Information
pCVD019 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCVD019 vector Sequence
LOCUS 40924_49107 4098 bp DNA circular SYN 18-DEC-2018
DEFINITION Broad host range vector vector pCVD019, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4098)
AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B,
Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
TITLE Broad-host-range vector system for synthetic biology and
biotechnology in cyanobacteria
JOURNAL Nucleic Acids Res. (2014) In press
PUBMED 25074377
REFERENCE 2 (bases 1 to 4098)
AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B,
Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
TITLE Direct Submission
JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University
of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE 3 (bases 1 to 4098)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4098)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res. (2014) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-JUN-2014) Division of Biological Sciences, University of
California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4098
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 46..51
/label=ZraI
/note="ZraI"
misc_feature 49..69
/note="G5C5; GC-adaptor for seamless assembly, flanking a
BHR replicons, neutral sites, or cyanobacterial replicons;
synthetic"
misc_feature 70..75
/label=XbaI
/note="XbaI"
misc_recomb 76..855
/note="S7942NS1-RA; right arm of the homologous
recombination site NS1 into the chromosome of Synechococcus
elongatus PCC7942 derived from INSD accession NC_007604,
U30252"
rep_origin 892..1480
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
oriT complement(1721..1830)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
CDS complement(2154..2600)
/gene="ndk"
/label=ndk
/note="Nucleoside diphosphate kinase from Synechococcus
elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805).
Accession#: P50590"
misc_feature 2606..2611
/label=XbaI
/note="XbaI"
misc_feature 2793..2798
/label=NheI
/note="NheI"
misc_feature 2853..2858
/label=EcoRV
/note="EcoRV"
misc_feature 2888..2893
/label=NheI
/note="NheI"
misc_feature 2894..2914
/note="GC; GC-adaptor for seamless assembly, flanking BHR
replicons or neutral sites; synthetic"
misc_feature 2912..2917
/label=ZraI
/note="ZraI"
CDS complement(3041..3898)
/label=AmpR
/note="beta-lactamase"
promoter complement(3899..4003)
/label=AmpR promoter
misc_feature 4029..4034
/label=ZraI
/note="ZraI"
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