Basic Vector Information
- Vector Name:
- pCVD005
- Antibiotic Resistance:
- Kanamycin
- Length:
- 3315 bp
- Type:
- Broad host range vector
- Replication origin:
- ori
- Source/Author:
- Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
- Promoter:
- tac
pCVD005 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pCVD005 vector Sequence
LOCUS 40924_49037 3315 bp DNA circular SYN 18-DEC-2018
DEFINITION Broad host range vector vector pCVD005, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3315)
AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B,
Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
TITLE Broad-host-range vector system for synthetic biology and
biotechnology in cyanobacteria
JOURNAL Nucleic Acids Res. (2014) In press
PUBMED 25074377
REFERENCE 2 (bases 1 to 3315)
AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B,
Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
TITLE Direct Submission
JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University
of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE 3 (bases 1 to 3315)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3315)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic
Acids Res. (2014) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(17-JUN-2014) Division of Biological Sciences, University of
California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3315
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 293..309
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 320..338
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 343..354
/label=Factor Xa site
/note="Factor Xa recognition and cleavage site"
misc_feature 370..375
/label=EcoRV
/note="EcoRV"
misc_feature complement(373..393)
/note="C2G; GC-adaptor for seamless assembly, flanking
antibiotic resistance markers, functional devices or custom
sequences; synthetic"
misc_feature 394..399
/label=AgeI
/note="AgeI"
promoter 406..434
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
regulatory 447..471
/note="luxA 5-UTR; ribosomal binding site"
/regulatory_class="promoter"
CDS 472..1329
/label=AmpR
/note="beta-lactamase"
regulatory 1341..1367
/note="trpA T; trpA terminator from Lorist6 INSD accession
X98450, one nt shorter than reference; Lorist6"
/regulatory_class="terminator"
misc_feature 1377..1382
/label=NheI
/note="NheI"
misc_feature complement(1383..1403)
/note="GC; GC-adaptor for seamless assembly, flanking
antibiotic resistance markers or Escherichia coli origins
to be assembled with a cyanobacterial replicon; synthetic"
misc_feature 1401..1406
/label=EcoRV
/note="EcoRV"
rep_origin complement(1631..2219)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2396..3187)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter complement(3188..3292)
/label=AmpR promoter
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