Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | vector pCVD004 | Antibiotic Resistance | Ampicillin |
| Length | 3659 bp | Type | Broad host range vector |
| Source | Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW. | ||
vector pCVD004 vector Vector Map
vector pCVD004 vector Sequence
LOCUS Exported 3659 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION Broad host range vector vector pCVD004, complete sequence.
ACCESSION KM017939
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3659)
AUTHORS Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B,
Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW.
TITLE Broad-host-range vector system for synthetic biology and
biotechnology in cyanobacteria
JOURNAL Nucleic Acids Res. (2014) In press
PUBMED 25074377
REFERENCE 2 (bases 1 to 3659)
AUTHORS Taton A, Unglaub F, Wright N, Zeng WY, Paz Yepes J, Brahamsha B,
Palenik B, Peterson T, Haerizadeh F, Golden SS, Golden JW.
TITLE Direct Submission
JOURNAL Submitted (17-JUN-2014) Division of Biological Sciences, University
of California, San Diego, 9500 Gilman Dr., La Jolla, CA 92093, USA
REFERENCE 3 (bases 1 to 3659)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3659
/organism="Broad host range vector vector pCVD004"
/mol_type="other DNA"
/note="bla_A7120 is a device that confers ampicillin (Ap)
resistance"
/db_xref="taxon:1531862"
primer_bind 293..309
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 320..338
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 370..375
/label=EcoRV
/note="EcoRV"
misc_feature complement(373..1684)
/note="CVD-ANTR; device for the GC-adaptor assembly:
antibiotic resistance markers"
misc_feature complement(373..393)
/note="C2G; GC-adaptor for seamless assembly, flanking
antibiotic resistance markers, functional devices or custom
sequences; synthetic"
misc_feature 394..399
/label=AgeI
/note="AgeI"
regulatory 400..539
/regulatory_class="promoter"
/note="PaadA; aadA 5'UTR, including promoter and RBS
conserved between pAM2178 and pcr8GWtopo"
CDS 540..1613
/codon_start=1
/product="bla_A7120"
/label=bla_A7120
/note="confers ampicillin resistance; codon optimized for
Anabaena sp. PCC7120; amino acid sequence derived from
pUC19"
/protein_id="AII99784.1"
/translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPSKQQARYA
VGRCLMLWSSNDVTQQGSRPKTKLNIMSIQHFRVALIPFFAAFCLPVFAHPETLVKVKD
AEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRR
IHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLH
NMGDHVTRLDRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWME
ADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMD
ERNRQIAEIGASLIKHW"
CDS 753..1613
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
regulatory 1622..1648
/regulatory_class="terminator"
/note="trpA T; trpA terminator from Lorist6 INSD accession
X98450, one nt shorter than reference; Lorist6"
misc_feature 1658..1663
/label=NheI
/note="NheI"
misc_feature complement(1664..1684)
/note="GC; GC-adaptor for seamless assembly, flanking
antibiotic resistance markers or Escherichia coli origins
to be assembled with a cyanobacterial replicon; synthetic"
misc_feature 1682..1687
/label=EcoRV
/note="EcoRV"
rep_origin complement(1853..2526)
/direction=LEFT
/note="colE1_pMA-RQ; origin of replication of colE1 found
inderived from pMA-RQ GeneART vector"
rep_origin complement(1912..2500)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2671..3531)
/codon_start=1
/product="bla_pMA-RQ"
/label=bla_pMA-RQ
/note="confers ampicillin resistance; beta-lactamase;
derived from pMA-RQ GeneART vector, has one point mutation
compared to pUC19"
/protein_id="AII99785.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3532..3636)
/gene="bla"
/label=AmpR promoter
regulatory complement(3532..3601)
/regulatory_class="promoter"
/note="Pbla; upstream region of the bla ORF including
promoter and RBS; derived from pUC19"
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