Basic Vector Information
Ubipro-STOP-FLAG-deltaPol vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Ubipro-STOP-FLAG-deltaPol vector Sequence
LOCUS 40924_49007 14503 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector Ubipro-STOP-FLAG-deltaPol, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 14503)
AUTHORS Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
Belyaeva ES, Belyakin SN.
TITLE Repressive histone marks renewal impedes replication fork
progression and contributes to late replication in Drosophila
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 14503)
AUTHORS Belyakin SN.
TITLE Direct Submission
JOURNAL Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave.
8/2, Novosibirsk, NSO 630090, Russia
REFERENCE 3 (bases 1 to 14503)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 14503)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 8/2,
Novosibirsk, NSO 630090, Russia"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..14503
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 224..2041
/label=Polyubiquitin
/note="Promoter of polyubiqutin gene of Drosophila
melanogaster"
protein_bind 2094..2127
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
polyA_signal 2154..3026
/label=hsp70 poly(A)
/note="polyadenlyation signal from the Drosophila
melanogaster hsp70Ab gene"
protein_bind complement(4211..4244)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
CDS 4304..4327
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
CDS 4349..5641
/gene="PolD2"
/label=PolD2
/note="DNA polymerase delta subunit 2 from Drosophila
melanogaster. Accession#: Q9W088"
intron 5805..5870
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 6000..6020
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
polyA_signal 6292..6426
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 10502..10571
/label=attB
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
misc_feature complement(10925..11510)
/label=P element 5' end
rep_origin complement(11745..12333)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12507..13364)
/label=AmpR
/note="beta-lactamase"
promoter complement(13365..13469)
/label=AmpR promoter
misc_feature complement(14271..14503)
/label=P element 3' end
/note="P element 3' end"
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