Ubipro-STOP-FLAG-deltaPol vector (V001581)

Basic Vector Information

      • Vector Name:
      • Ubipro-STOP-FLAG-deltaPol
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 14503 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, Belyaeva ES, Belyakin SN.

Ubipro-STOP-FLAG-deltaPol vector Vector Map

Ubipro-STOP-FLAG-deltaPol14503 bp7001400210028003500420049005600630070007700840091009800105001120011900126001330014000loxPhsp70 poly(A)loxPFLAG-deltaPolsmall t intronSV40 NLSSV40 poly(A) signalattBP element 5' endoriAmpRAmpR promoterP element 3' end

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Ubipro-STOP-FLAG-deltaPol vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               14503 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector Ubipro-STOP-FLAG-deltaPol, complete sequence.
ACCESSION   KT921802
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, 
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive histone marks renewal impedes replication fork 
            progression and contributes to late replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14503)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 14503)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Cloning vector Ubipro-STOP-FLAG-deltaPol"
                     /mol_type="other DNA"
                     /db_xref="taxon:1816698"
     regulatory      40..2043
                     /regulatory_class="promoter"
     protein_bind    2094..2127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    2154..3026
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     protein_bind    4211..4244
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             4301..5644
                     /codon_start=1
                     /product="FLAG-deltaPol"
                     /label=FLAG-deltaPol
                     /protein_id="AMR68903.1"
                     /translation="MDYKDDDDKSRNIDGIMTNRLETKYENLSSIFRLKGHDYQKQFFH
                     LYAHRLAEMTRLLTPLAQKKWGNKEPIKKLCELRGEQDVHCILIGTIFKHQAHKPSILR
                     DISEENQLAPQPPRQNYSDPEDKIVLEDELQRVRLQGKLNGQLLATGVVCAALGGTDSD
                     GFFNVEDILFYESGPQKPLAPIKRSRLLVLASGLDQLQAHKFVEALNLFQYWLAGSLGN
                     NKEAGSMVRLIVAGNSVRASAMAHVPTLQVARTQANANDTVQAVSQLDQWFASWARSLP
                     VDIMPGPYDPANFMLPQQPFHKCMFPQAAQLATFQAVTNPYSCRLDEALVVGTSGQNVS
                     DLLRSTSLDSALEALRCTLTWGHVAPTAPDTLACYPYIESDPFILRECPHVYYAGNCES
                     FATELHEGSEGKRTRLVCVPSFSKTQSVAVVDLDTLDCRMVNFSVDAE"
     CDS             4304..4327
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          5805..5870
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6000..6020
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6292..6426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    10502..10571
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    10925..11510
                     /label=P element 5' end
     rep_origin      complement(11745..12333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12504..13364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(13365..13469)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    14271..14503
                     /label=P element 3' end
                     /note="P element 3' end"

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