Ubipro-STOP-FLAG-deltaPol vector (V001581)

LOCUS       V001581                14503 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001581
VERSION     V001581
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive histone marks renewal impedes replication fork
            progression and contributes to late replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14503)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave.
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 14503)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14503)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 8/2,
            Novosibirsk, NSO 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        224..2041
                     /label="Polyubiquitin"
                     /note="Promoter of polyubiqutin gene of Drosophila
                     melanogaster"
     protein_bind    2094..2127
                     /label="loxP"
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (GCATACAT)."
     polyA_signal    2154..3026
                     /label="hsp70 poly(A)"
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     protein_bind    complement(4211..4244)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4304..4327
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             4349..5641
                     /gene="PolD2"
                     /label="DNA polymerase delta subunit 2"
                     /note="DNA polymerase delta subunit 2 from Drosophila
                     melanogaster. Accession#: Q9W088"
     intron          5805..5870
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6000..6020
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    6292..6426
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    10502..10571
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     misc_feature    complement(10925..11510)
                     /label="P element 5' end"
     rep_origin      complement(11745..12333)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12507..13364)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13365..13469)
                     /label="AmpR promoter"
     misc_feature    complement(14271..14503)
                     /label="P element 3' end"
                     /note="P element 3' end"