Ubi-stop-mCD8GFP vector (V001582)

Basic Vector Information

      • Vector Name:
      • Ubi-stop-mCD8GFP
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 14482 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Laktionov P, Maksimov D, White-Cooper H, Belyakin S.

Ubi-stop-mCD8GFP vector Vector Map

Ubi-stop-mCD8GFP14482 bp7001400210028003500420049005600630070007700840091009800105001120011900126001330014000Drosophila melanogaster Ubi-p63E gene promoterloxP recombination sitehsp70Ab transcription terminatorloxP recombination sitemCD8-GFP fusionsmall t intronSV40 NLSSV40 poly(A) signalmini-whitephiC31 attB siteP element 5' endoriblaAmpR promoterP element 3' end

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Ubi-stop-mCD8GFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported               14482 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector Ubi-stop-mCD8GFP, complete sequence.
ACCESSION   KC845568
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Laktionov P, Maksimov D, White-Cooper H, Belyakin S.
  TITLE     Cookie monster binding to the repressed chromosomal domains is 
            needed for selective activation of testis-specific genes in 
            Drosophila melanogaster
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14482)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2013) Genomics lab, Institute of molecular and 
            cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia
REFERENCE   3  (bases 1 to 14482)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Cloning vector Ubi-stop-mCD8GFP"
                     /mol_type="other DNA"
                     /db_xref="taxon:1366865"
     regulatory      40..2041
                     /regulatory_class="promoter"
                     /gene="Ubi-p63E"
                     /note="Drosophila melanogaster Ubi-p63E gene promoter"
     gene            40..2041
                     /gene="Ubi-p63E"
                     /label=Ubi-p63E
     misc_recomb     2094..2127
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    2094..2127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      2146..4178
                     /regulatory_class="terminator"
                     /note="hsp70Ab transcription terminator"
     polyA_signal    2154..3026
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila 
                     melanogaster hsp70Ab gene"
     misc_recomb     4210..4243
                     /label=loxP recombination site
                     /note="loxP recombination site"
     protein_bind    4210..4243
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             4312..5700
                     /codon_start=1
                     /gene="mCD8-GFP fusion"
                     /product="mCD8-GFP fusion protein"
                     /label=mCD8-GFP fusion
                     /protein_id="AGR55450.1"
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLI?TLICYHSR
                     GSMSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE?TIFFKDDGNYKTRAEVKFEG
                     DTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGS
                     VQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMD
                     ELYK"
     gene            4312..5700
                     /gene="mCD8-GFP fusion"
                     /label=mCD8-GFP fusion
     CDS             4312..4977
                     /codon_start=1
                     /product="mouse lymphocyte antigen CD8 alpha chain"
                     /label=mCD8
                     /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL
                     GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA
                     MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP
                     VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLI?TLICYHSR
                     "
     CDS             4984..5700
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQE?TIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     intron          5784..5849
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5979..5999
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6271..6405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            6757..10302
                     /gene="mini-white"
                     /label=mini-white
     misc_recomb     10455..10778
                     /label=phiC31 attB site
                     /note="phiC31 attB site"
     protein_bind    10481..10550
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    10904..11489
                     /label=P element 5' end
     rep_origin      complement(11724..12312)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12483..13343)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="AGR55451.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(12483..13343)
                     /gene="bla"
                     /label=bla
     promoter        complement(13344..13448)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    14250..14482
                     /label=P element 3' end
                     /note="P element 3' end"

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