Basic Vector Information
Ubi-stop-mCD8GFP vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Ubi-stop-mCD8GFP vector Sequence
LOCUS 40924_49002 14482 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector Ubi-stop-mCD8GFP, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 14482) AUTHORS Laktionov P, Maksimov D, White-Cooper H, Belyakin S. TITLE Cookie monster binding to the repressed chromosomal domains is needed for selective activation of testis-specific genes in Drosophila melanogaster JOURNAL Unpublished REFERENCE 2 (bases 1 to 14482) AUTHORS Belyakin SN. TITLE Direct Submission JOURNAL Submitted (29-MAR-2013) Genomics lab, Institute of molecular and cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia REFERENCE 3 (bases 1 to 14482) TITLE Direct Submission REFERENCE 4 (bases 1 to 14482) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (29-MAR-2013) Genomics lab, Institute of molecular and cellular biology, Lavrentiev ave 8/2, Novosibirsk 630090, Russia" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..14482 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 224..2041 /label=Polyubiquitin /note="Promoter of polyubiqutin gene of Drosophila melanogaster" misc_recomb 2094..2127 /label=loxP recombination site /note="loxP recombination site" protein_bind 2094..2127 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." polyA_signal 2154..3026 /label=hsp70 poly(A) /note="polyadenlyation signal from the Drosophila melanogaster hsp70Ab gene" protein_bind complement(4210..4243) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." CDS 4312..4977 /codon_start=1 /product="mouse lymphocyte antigen CD8 alpha chain" /label=mCD8 /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIXTLICYHSR " CDS 4984..5697 /codon_start=1 /label=GFP /note="green fluorescent protein" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQEXTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITHGMDELYK" intron 5784..5849 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 5979..5999 /codon_start=1 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" polyA_signal 6271..6405 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" gene 6757..10302 /gene="mini-white" /label=mini-white protein_bind 10481..10550 /label=attB /note="attB site for the phi-C31 integrase (Groth et al., 2000)" misc_feature complement(10904..11489) /label=P element 5' end rep_origin complement(11724..12312) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(12486..13343) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(13344..13448) /label=AmpR promoter misc_feature complement(14250..14482) /label=P element 3' end /note="P element 3' end"
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