Basic Vector Information
SV40p TV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
SV40p TV vector Sequence
LOCUS 40924_48867 6588 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector SV40p TV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6588)
AUTHORS Konishi Y, Karnan S, Takahashi M, Ota A, Damdindorj L, Hosokawa Y,
Konishi H.
TITLE A system for the measurement of gene targeting efficiency in human
cell lines using an antibiotic resistance-GFP fusion gene
JOURNAL BioTechniques 53 (3), 141-152 (2012)
PUBMED 22963476
REFERENCE 2 (bases 1 to 6588)
AUTHORS Konishi H.
TITLE Direct Submission
JOURNAL Submitted (07-JAN-2012) Aichi Medical University School of Medicine,
1-1 Yazako-Karimta, Nagakute, Aichi 480-1195, Japan
REFERENCE 3 (bases 1 to 6588)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6588)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"BioTechniques"; date: "2012"; volume: "53"; issue: "3"; pages:
"141-152"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-JAN-2012) Aichi Medical University School of Medicine, 1-1
Yazako-Karimta, Nagakute, Aichi 480-1195, Japan"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6588
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
CDS 180..896
/label=EGFP
/note="enhanced GFP"
polyA_signal 912..1136
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind 1180..1213
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
promoter 1366..1723
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 1774..2565
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 2632..2680
/label=poly(A) signal
/note="synthetic polyadenylation signal"
misc_feature 2743..2776
/label=loxP
/note="loxP"
protein_bind complement(2743..2776)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind complement(2803..2819)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 2822..3840
/note="partial SV40 largeT; an internal portion of SV40
largeT exon 2"
repeat_region 3851..3991
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4066..4521
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4803..4907
/label=AmpR promoter
CDS 4908..5765
/label=AmpR
/note="beta-lactamase"
rep_origin 5939..6527
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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