SV40p TV vector (V001593)

Basic Vector Information

      • Vector Name:
      • SV40p TV
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6588 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Konishi Y, Karnan S, Takahashi M, Ota A, Damdindorj L, Hosokawa Y, Konishi H.

SV40p TV vector Vector Map

SV40p TV6588 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300ITREGFPbGH poly(A) signalloxPSV40 enhancer and early promoterneomycin resistance genesynthetic polyAloxPKS primerpartial SV40 largeT; an internal portion of SV40 largeT exon 2ITRf1 oriAmpR promoterampRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

SV40p TV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6588 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector SV40p TV, complete sequence.
ACCESSION   JQ394984
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Konishi Y, Karnan S, Takahashi M, Ota A, Damdindorj L, Hosokawa Y, 
            Konishi H.
  TITLE     A system for the measurement of gene targeting efficiency in human 
            cell lines using an antibiotic resistance-GFP fusion gene
  JOURNAL   BioTechniques 53 (3), 141-152 (2012)
  PUBMED    22963476
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Konishi H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2012) Aichi Medical University School of Medicine,
            1-1 Yazako-Karimta, Nagakute, Aichi 480-1195, Japan
REFERENCE   3  (bases 1 to 6588)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Cloning vector SV40p TV"
                     /mol_type="other DNA"
                     /db_xref="taxon:1249022"
     misc_feature    1..141
                     /label=ITR
                     /note="ITR"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     gene            181..896
                     /gene="EGFP"
                     /label=EGFP
     CDS             183..899
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             183..896
                     /codon_start=1
                     /gene="EGFP"
                     /product="EGFP"
                     /label=EGFP
                     /protein_id="AFV73973.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    912..1136
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    913..1136
                     /label=bovine growth hormone polyA
                     /note="bovine growth hormone polyA"
     misc_feature    1180..1213
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(1180..1213)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      1311..1729
                     /regulatory_class="promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        1366..1723
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1574..1709
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1774..2568
                     /codon_start=1
                     /product="neomycin phosphotransferase"
                     /label=neomycin phosphotransferase
                     /note="neomycin resistance gene"
                     /protein_id="AFV73972.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    2632..2680
                     /label=synthetic polyA
                     /note="synthetic polyA"
     polyA_signal    2632..2680
                     /note="synthetic polyadenylation signal"
     misc_feature    2743..2776
                     /label=loxP
                     /note="loxP"
     protein_bind    complement(2743..2776)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     complement(2803..2819)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2822..3840
                     /note="partial SV40 largeT; an internal portion of SV40 
                     largeT exon 2"
     misc_feature    3851..3991
                     /label=ITR
                     /note="ITR"
     repeat_region   3851..3991
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4066..4521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4803..4907
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4908..5768
                     /codon_start=1
                     /gene="ampR"
                     /product="beta-lactamase"
                     /label=ampR
                     /note="ampicilin resistance protein"
                     /protein_id="AFV73971.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            4908..5768
                     /gene="ampR"
                     /label=ampR
     rep_origin      5939..6527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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