Sos_ZsG_pol I-mg vector (V001594)

LOCUS       40924_48818        3896 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector Sos_ZsG_pol I-mg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Welch SR, Chakrabarti AK, Wiggleton Guerrero L, Jenks HM, Lo MK, 
            Nichol ST, Spiropoulou CF, Albarino CG.
  TITLE     Development of a reverse genetics system for Sosuga virus allows 
            rapid screening of antiviral compounds
  JOURNAL   PLoS Negl Trop Dis 12 (3), e0006326 (2018)
  PUBMED    29522528
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Albarino C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Viral Special Pathogens Branch, Centers for 
            Disease Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 
            30329, USA
REFERENCE   3  (bases 1 to 3896)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis"; date: "2018"; volume: "12"; issue: "3"; pages: "e0006326"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-JAN-2018) Viral Special Pathogens Branch, Centers for Disease 
            Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 30329, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2..35
                     /label=pol I terminator
                     /note="pol I terminator"
                     /regulatory_class="terminator"
     CDS             200..892
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     regulatory      complement(962..1173)
                     /label=pol I promoter
                     /note="pol I promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(1244..1260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1268..1284)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1292..1322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1337..1358)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1646..2234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2408..3265)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3266..3370)
                     /label=AmpR promoter
     primer_bind     3844..3860
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"