Basic Vector Information
- Vector Name:
- Sos_ZsG_pol I-mg
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3896 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Welch SR, Chakrabarti AK, Wiggleton Guerrero L, Jenks HM, Lo MK, Nichol ST, Spiropoulou CF, Albarino CG.
Sos_ZsG_pol I-mg vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Sos_ZsG_pol I-mg vector Sequence
LOCUS 40924_48818 3896 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector Sos_ZsG_pol I-mg, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3896)
AUTHORS Welch SR, Chakrabarti AK, Wiggleton Guerrero L, Jenks HM, Lo MK,
Nichol ST, Spiropoulou CF, Albarino CG.
TITLE Development of a reverse genetics system for Sosuga virus allows
rapid screening of antiviral compounds
JOURNAL PLoS Negl Trop Dis 12 (3), e0006326 (2018)
PUBMED 29522528
REFERENCE 2 (bases 1 to 3896)
AUTHORS Albarino C.
TITLE Direct Submission
JOURNAL Submitted (30-JAN-2018) Viral Special Pathogens Branch, Centers for
Disease Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA
30329, USA
REFERENCE 3 (bases 1 to 3896)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3896)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl
Trop Dis"; date: "2018"; volume: "12"; issue: "3"; pages: "e0006326"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(30-JAN-2018) Viral Special Pathogens Branch, Centers for Disease
Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 30329, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3896
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 2..35
/label=pol I terminator
/note="pol I terminator"
/regulatory_class="terminator"
CDS 200..892
/label=ZsGreen1
/note="Zoanthus green fluorescent protein"
regulatory complement(962..1173)
/label=pol I promoter
/note="pol I promoter"
/regulatory_class="promoter"
primer_bind complement(1244..1260)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1268..1284)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1292..1322)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1337..1358)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1646..2234)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2408..3265)
/label=AmpR
/note="beta-lactamase"
promoter complement(3266..3370)
/label=AmpR promoter
primer_bind 3844..3860
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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