Sos_ZsG_pol I-mg vector (V001594)

Basic Vector Information

      • Vector Name:
      • Sos_ZsG_pol I-mg
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3896 bp
      • Type:
      • Expression vector
      • Source/Author:
      • Welch SR, Chakrabarti AK, Wiggleton Guerrero L, Jenks HM, Lo MK, Nichol ST, Spiropoulou CF, Albarino CG.

Sos_ZsG_pol I-mg vector Vector Map

Sos_ZsG_pol I-mg3896 bp60012001800240030003600pol I terminatorSOSV/ZsG minigenomepol I promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterM13 fwd

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Sos_ZsG_pol I-mg vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3896 bp ds-DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector Sos_ZsG_pol I-mg, complete sequence.
ACCESSION   MG880223
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Welch SR, Chakrabarti AK, Wiggleton Guerrero L, Jenks HM, Lo MK, 
            Nichol ST, Spiropoulou CF, Albarino CG.
  TITLE     Development of a reverse genetics system for Sosuga virus allows 
            rapid screening of antiviral compounds
  JOURNAL   PLoS Negl Trop Dis 12 (3), e0006326 (2018)
  PUBMED    29522528
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Albarino C.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JAN-2018) Viral Special Pathogens Branch, Centers for 
            Disease Control and Prevention, 1600 Clifton Rd. NE, Atlanta, GA 
            30329, USA
REFERENCE   3  (bases 1 to 3896)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Expression vector Sos_ZsG_pol I-mg"
                     /focus
                     /mol_type="other DNA"
                     /db_xref="taxon:2126431"
     source          36..960
                     /organism="Sosuga virus"
                     /mol_type="other DNA"
                     /db_xref="taxon:1452514"
     regulatory      2..35
                     /regulatory_class="terminator"
                     /note="pol I terminator"
     misc_feature    36..960
                     /label=SOSV/ZsG minigenome
                     /note="SOSV/ZsG minigenome"
     CDS             200..895
                     /codon_start=1
                     /product="ZsG"
                     /label=ZsG
                     /protein_id="AVT50845.1"
                     /translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
                     LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
                     CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
                     DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
                     AIASGSALP"
     regulatory      complement(962..1173)
                     /regulatory_class="promoter"
                     /note="pol I promoter"
     primer_bind     complement(1244..1260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1268..1284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1292..1322)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1337..1358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(1646..2234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2405..3265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3266..3370)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3844..3860
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"

This page is informational only.