SBa_000560 vector (V001598)

LOCUS       V001598                 9859 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V001598
VERSION     V001598
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Temme K, Zhao D, Voigt CA.
  TITLE     Refactoring the nitrogen fixation gene cluster from Klebsiella
            oxytoca
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 109 (18), 7085-7090 (2012)
   PUBMED   22509035
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Temme K, Zhao D, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2012) Biological Engineering, MIT, 500 Technology
            Square, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 9859)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9859)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2012"; volume: "109"; issue: "18"; pages:
            "7085-7090"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-APR-2012) Biological Engineering, MIT, 500 Technology Square,
            Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1..60
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     terminator      complement(120..149)
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     misc_feature    196..217
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     CDS             complement(255..1334)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(1335..1412)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     protein_bind    1963..1979
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    1989..2028
                     /label="SBa_000508"
                     /note="SBa_000508"
     regulatory      2029..2062
                     /label="SBa_000507"
                     /note="SBa_000507"
                     /regulatory_class="ribosome_binding_site"
     regulatory      2063..2188
                     /label="SBa_000509"
                     /note="SBa_000509"
                     /regulatory_class="other"
     CDS             2189..4834
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     misc_feature    4838..4858
                     /label="BioBrick suffix"
                     /note="universal suffix for all parts"
     terminator      4894..4925
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     regulatory      4973..5037
                     /label="SBa_000488"
                     /note="SBa_000488"
                     /regulatory_class="terminator"
     CDS             5787..6755
                     /label="RepA"
                     /note="replication protein of plasmid pSa"
     rep_origin      6795..7230
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             8911..9699
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"