Basic Vector Information
- Vector Name:
- SBa_000560
- Antibiotic Resistance:
- Streptomycin
- Length:
- 9859 bp
- Type:
- Cloning vector
- Replication origin:
- pSa ori
- Source/Author:
- Temme K, Zhao D, Voigt CA.
SBa_000560 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
SBa_000560 vector Sequence
LOCUS 40924_48758 9859 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector SBa_000560, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9859)
AUTHORS Temme K, Zhao D, Voigt CA.
TITLE Refactoring the nitrogen fixation gene cluster from Klebsiella
oxytoca
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 109 (18), 7085-7090 (2012)
PUBMED 22509035
REFERENCE 2 (bases 1 to 9859)
AUTHORS Temme K, Zhao D, Voigt CA.
TITLE Direct Submission
JOURNAL Submitted (05-APR-2012) Biological Engineering, MIT, 500 Technology
Square, Cambridge, MA 02139, USA
REFERENCE 3 (bases 1 to 9859)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9859)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
Acad. Sci. U.S.A."; date: "2012"; volume: "109"; issue: "18"; pages:
"7085-7090"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(05-APR-2012) Biological Engineering, MIT, 500 Technology Square,
Cambridge, MA 02139, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..9859
/mol_type="other DNA"
/organism="synthetic DNA construct"
terminator 1..60
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
terminator complement(120..149)
/label=T3Te terminator
/note="phage T3 early transcription terminator"
misc_feature 196..217
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
'ATG'"
CDS complement(255..1334)
/label=lacI
/note="lac repressor"
promoter complement(1335..1412)
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
protein_bind 1963..1979
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 1989..2028
/label=SBa_000508
/note="SBa_000508"
regulatory 2029..2062
/label=SBa_000507
/note="SBa_000507"
/regulatory_class="ribosome_binding_site"
regulatory 2063..2188
/label=SBa_000509
/note="SBa_000509"
/regulatory_class="other"
CDS 2189..4834
/note="T7 RNA polymerase from Escherichia phage T7.
Accession#: P00573"
misc_feature 4838..4858
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 4894..4925
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
regulatory 4973..5037
/label=SBa_000488
/note="SBa_000488"
/regulatory_class="terminator"
CDS 5787..6755
/label=RepA
/note="replication protein of plasmid pSa"
rep_origin 6795..7230
/label=pSa ori
/note="origin of replication from bacterial plasmid pSa"
CDS 8911..9699
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
This page is informational only.