Basic Vector Information
DsRed-Rab5 WT vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
DsRed-Rab5 WT vector Sequence
LOCUS DsRed-Rab5_WT. 5369 bp DNA circular SYN 13-MAY-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS DsRed-Rab5 WT. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5369) AUTHORS Sharma DK, Choudhury A, Singh RD, Wheatley CL, Marks DL, Pagano RE TITLE Glycosphingolipids internalized via caveolar-related endocytosis rapidly merge with the clathrin pathway in early endosomes and form microdomains for recycling. JOURNAL J Biol Chem. 2003 Feb 28. 278(9):7564-72. PUBMED 12482757 REFERENCE 2 (bases 1 to 5369) TITLE Direct Submission REFERENCE 3 (bases 1 to 5369) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol Chem. 2003 Feb 28. 278(9):7564-72." COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..5369 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 16..319 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 320..523 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" CDS 568..1242 /label=DsRed2 /note="improved tetrameric variant of DsRed fluorescent protein" regulatory 1249..1258 /label=Kozak sequence /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 1261..1287 /label=HA /note="HA (human influenza hemagglutinin) epitope tag" CDS 1291..1932 /gene="RAB5A" /label=RAB5A /note="Ras-related protein Rab-5A from Homo sapiens. Accession#: P20339" misc_feature 1936..1988 /label=MCS /note="multiple cloning site" polyA_signal 2112..2233 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(2240..2695) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 2722..2826 /label=AmpR promoter promoter 2828..3185 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 3220..4011 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" primer_bind complement(4202..4221) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4246..4293 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4622..5210 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 5325..5369 /label=CMV /note="Human cytomegalovirus immediate early enhancer/promoter"
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