DsRed-rab7 WT vector (V001733)

Basic Vector Information

      • Vector Name:
      • DsRed-rab7 WT
      • Antibiotic Resistance:
      • Kanamycin
      • Length:
      • 6060 bp
      • Type:
      • Mammalian Expression
      • Replication origin:
      • ori
      • Selection Marker:
      • Neomycin (select with G418)
      • Copy Number:
      • High Copy
      • Promoter:
      • CMV
      • Cloning Method:
      • Restriction Enzyme
      • 5' Primer:
      • DsRed1-C
      • 3' Primer:
      • SV40pA-R

DsRed-rab7 WT vector Vector Map

DsRed-rab7 WT6060 bp30060090012001500180021002400270030003300360039004200450048005100540057006000CMV enhancerCMV promoterDsRed1MCSHAHARAB7AHAHARAB7ASV40 poly(A) signalf1 oriAmpR promoterSV40 promoterNeoR/KanRTK-pA-RHSV TK poly(A) signalori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

DsRed-rab7 WT vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       DsRed-rab7_WT.        6060 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
KEYWORDS    DsRed-rab7 WT.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL, 
            Marks DL, Pagano RE
  TITLE     Rab proteins mediate Golgi transport of caveola-internalized 
            glycosphingolipids and correct lipid trafficking in Niemann-Pick C 
  JOURNAL   J Clin Invest. 2002 Jun . 109(12):1541-50.
  PUBMED    12070301
REFERENCE   2  (bases 1 to 6060)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 6060)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2002 Jun . 109(12):1541-50."
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        372..575
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
     CDS             620..1297
                     /note="wild-type DsRed"
     misc_feature    1301..1357
                     /note="multiple cloning site"
     CDS             1361..1387
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1388..1414
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             1418..2035
                     /note="Ras-related protein Rab-7a from Homo sapiens.
                     Accession#: P51149"
     CDS             2048..2074
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2075..2101
                     /note="HA (human influenza hemagglutinin) epitope tag"
     CDS             2105..2722
                     /note="Ras-related protein Rab-7a from Homo sapiens.
                     Accession#: P51149"
     polyA_signal    2855..2976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2983..3438)
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3465..3569
                     /label=AmpR promoter
     promoter        3571..3928
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3963..4754
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(4945..4964)
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4989..5036
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5365..5953
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 

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