Basic Vector Information
- Vector Name:
- DsRed-rab7 WT
- Antibiotic Resistance:
- Kanamycin
- Length:
- 6060 bp
- Type:
- Mammalian Expression
- Replication origin:
- ori
- Selection Marker:
- Neomycin (select with G418)
- Copy Number:
- High Copy
- Promoter:
- CMV
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- DsRed1-C
- 3' Primer:
- SV40pA-R
DsRed-rab7 WT vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
DsRed-rab7 WT vector Sequence
LOCUS DsRed-rab7_WT. 6060 bp DNA circular SYN 13-MAY-2021
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS DsRed-rab7 WT.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6060)
AUTHORS Choudhury A, Dominguez M, Puri V, Sharma DK, Narita K, Wheatley CL,
Marks DL, Pagano RE
TITLE Rab proteins mediate Golgi transport of caveola-internalized
glycosphingolipids and correct lipid trafficking in Niemann-Pick C
cells.
JOURNAL J Clin Invest. 2002 Jun . 109(12):1541-50.
PUBMED 12070301
REFERENCE 2 (bases 1 to 6060)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6060)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Clin
Invest. 2002 Jun . 109(12):1541-50."
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6060
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 68..371
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 372..575
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 620..1297
/label=DsRed1
/note="wild-type DsRed"
misc_feature 1301..1357
/label=MCS
/note="multiple cloning site"
CDS 1361..1387
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 1388..1414
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 1418..2035
/gene="RAB7A"
/label=RAB7A
/note="Ras-related protein Rab-7a from Homo sapiens.
Accession#: P51149"
CDS 2048..2074
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 2075..2101
/label=HA
/note="HA (human influenza hemagglutinin) epitope tag"
CDS 2105..2722
/gene="RAB7A"
/label=RAB7A
/note="Ras-related protein Rab-7a from Homo sapiens.
Accession#: P51149"
polyA_signal 2855..2976
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2983..3438)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3465..3569
/label=AmpR promoter
promoter 3571..3928
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3963..4754
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
primer_bind complement(4945..4964)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4989..5036
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5365..5953
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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