Basic Vector Information
- Vector Name:
- pMXs-c-Myc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6087 bp
- Type:
- Mammalian Expression, Retroviral
- Replication origin:
- ori
- Copy Number:
- High Copy
- Cloning Method:
- Restriction Enzyme
- 5' Primer:
- pMX-S1811
pMXs-c-Myc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMXs-c-Myc vector Sequence
LOCUS 40924_32863 6087 bp DNA circular SYN 13-MAY-2021
DEFINITION Retroviral expression of mouse c-Myc.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6087)
AUTHORS Takahashi K, Yamanaka S
TITLE Induction of pluripotent stem cells from mouse embryonic and adult
fibroblast cultures by defined factors.
JOURNAL Cell. 2006 Aug 25. 126(4):663-76.
PUBMED 16904174
REFERENCE 2 (bases 1 to 6087)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 6087)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006
Aug 25. 126(4):663-76."
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6087
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR complement(120..711)
/label=LTR
/note="long terminal repeat from Moloney murine leukemia
virus"
protein_bind 906..930
/label=attB2
/note="recombination site for the Gateway(R) BP reaction"
CDS complement(950..2269)
/codon_start=1
/label=mMyc
/note="Mus musculus myc proto-oncogene. Belongs to
myelocytomatosis (Myc) family of transcription factors.
Plays a role in cell cycle procession, apotosis and
cellular transformation"
/translation="TMPLNVNFTNRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPA
PSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMM
TELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTS
LSPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKSCTSSDSTAFSPSS
DSLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQTPAKRSESG
SSPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNN
RKCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKK
ATAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA"
protein_bind complement(2287..2311)
/label=attB1
/note="recombination site for the Gateway(R) BP reaction"
misc_feature complement(2362..2736)
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
CDS complement(2746..3162)
/codon_start=1
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
/translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
misc_feature complement(3221..3578)
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
promoter 4189..4293
/label=AmpR promoter
CDS 4294..5151
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5318..5906
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6060..6077
/label=L4440
/note="L4440 vector, forward primer"
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