Basic Vector Information
- Vector Name:
- P{attP.w+.attP}
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8266 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Bateman JR, Wu CT.
P{attP.w+.attP} vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
P{attP.w+.attP} vector Sequence
LOCUS 40924_48643 8266 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector P{attP.w+.attP}, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8266)
AUTHORS Bateman JR, Wu CT.
TITLE A simple polymerase chain reaction-based method for the construction
of recombinase-mediated cassette exchange donor vectors
JOURNAL Genetics 180 (3), 1763-1766 (2008)
PUBMED 18791223
REFERENCE 2 (bases 1 to 8266)
AUTHORS Bateman JR.
TITLE Direct Submission
JOURNAL Submitted (15-MAY-2008) Genetics, Harvard Medical School, 77 Avenue
Louis Pasteur, Boston, MA 02115, USA
REFERENCE 3 (bases 1 to 8266)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8266)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genetics";
date: "2008"; volume: "180"; issue: "3"; pages: "1763-1766"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-MAY-2008) Genetics, Harvard Medical School, 77 Avenue Louis
Pasteur, Boston, MA 02115, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8266
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature complement(1..233)
/label=P element 3' end
/note="P element 3' end"
protein_bind 365..464
/label=phage phi-C31 attP
/note="attachment site of phage phi-C31"
misc_feature 549..4569
/label=mini-white dominant eye marker
/note="mini-white dominant eye marker"
CDS join(1035..1106,1501..1774,1849..2503,2565..2880,3101..3232,
3303..3917)
/codon_start=1
/gene="white"
/product="Drosophila white gene eye color pigment"
/label=mini-white
/note="This is a modified version of the white gene lacking
part of the first intron."
/translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
protein_bind complement(4632..4731)
/label=phage phi-C31 attP
/note="attachment site of phage phi-C31"
misc_feature complement(4921..5506)
/label=P element 5' end
rep_origin complement(5741..6329)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6503..7360)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7361..7465)
/label=AmpR promoter
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