Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | pwFRT-PCS12-Tp | Antibiotic Resistance | Ampicillin |
| Length | 3728 bp | Type | FRT-Tp-FRT expression vector |
| Source | Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT. | ||
pwFRT-PCS12-Tp vector Vector Map
pwFRT-PCS12-Tp vector Sequence
LOCUS Exported 3728 bp ds-DNA circular SYN 18-DEC-2018
DEFINITION FRT-Tp-FRT expression vector pwFRT-PCS12-Tp, complete sequence.
ACCESSION EU334849
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3728)
AUTHORS Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT.
TITLE Genetic tools for allelic replacement in Burkholderia species
JOURNAL Appl. Environ. Microbiol. 74 (14), 4498-4508 (2008)
PUBMED 18502918
REFERENCE 2 (bases 1 to 3728)
AUTHORS Barrett AR, Kang Y, Vukovich JM, Son MS, Hoang TT.
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2007) Microbiology, University of Hawaii at Manoa,
2538 McCarthy Mall Snyder Hall Rm 310, Honolulu, HI 96822, USA
REFERENCE 3 (bases 1 to 3728)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3728
/organism="FRT-Tp-FRT expression vector pwFRT-PCS12-Tp"
/mol_type="other DNA"
/db_xref="taxon:491856"
promoter 96..200
/gene="bla"
/label=AmpR promoter
CDS 201..1061
/codon_start=1
/gene="bla"
/product="Bla"
/label=bla
/note="beta-lactamase"
/protein_id="ABY61790.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
gene 201..1061
/gene="bla"
/label=bla
rep_origin 1232..1820
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
rep_origin 1268..1884
/label=ColE1 origin
/note="ColE1 origin"
protein_bind 2108..2129
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2144..2174
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2182..2198
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 2206..2222
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_recomb 2289..2362
/note="FRT; Flp-recombination-target"
protein_bind complement(2306..2353)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
regulatory 2393..2434
/regulatory_class="enhancer"
/note="Burkholderia cenocepacia S12 promoter"
RBS 2445..2467
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
promoter 2565..2593
/gene="intI1 (promoter lies within the coding sequence)"
/label=Pc promoter
/note="class 1 integron promoter"
CDS 2920..3156
/codon_start=1
/gene="dhfr"
/product="DhfrII"
/label=dhfr
/note="dihydrofolate reductase protein"
/protein_id="ABY61791.1"
/translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
gene 2920..3156
/gene="dhfr"
/label=dhfr
misc_recomb 3203..3276
/note="FRT; Flp-recombination-target"
protein_bind complement(3220..3267)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
primer_bind complement(3334..3350)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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