pWFRT-mer vector (V002140)

LOCUS       V002140                 5440 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002140
VERSION     V002140
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT.
  TITLE     Genetic tools for allelic replacement in Burkholderia species
  JOURNAL   Appl. Environ. Microbiol. 74 (14), 4498-4508 (2008)
   PUBMED   18502918
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Barrett AR, Kang Y, Vukovich JM, Son MS, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2007) Microbiology, University of Hawaii at Manoa,
            2538 McCarthy Mall Snyder Hall Rm. 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 5440)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5440)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "14";
            pages: "4498-4508"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (04-DEC-2007) Microbiology, University of Hawaii at Manoa, 2538
            McCarthy Mall Snyder Hall Rm. 310, Honolulu, HI 96822, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label="AmpR promoter"
     CDS             201..1058
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2108..2129
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2289..2362
                     /label="Flip recognition target"
                     /note="Flip recognition target"
     protein_bind    complement(2306..2353)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     regulatory      2393..2434
                     /regulatory_class="promoter"
     RBS             2445..2467
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2474..2821
                     /gene="merT"
                     /label="Mercuric transport protein MerT"
                     /note="Mercuric transport protein MerT from Pseudomonas
                     aeruginosa. Accession#: P04140"
     CDS             2837..3109
                     /gene="merP"
                     /label="Mercuric transport protein periplasmic component"
                     /note="Mercuric transport protein periplasmic component
                     from Pseudomonas aeruginosa. Accession#: P04131"
     CDS             3184..4866
                     /gene="merA"
                     /label="Mercuric reductase"
                     /note="Mercuric reductase from Pseudomonas aeruginosa.
                     Accession#: P00392"
     protein_bind    complement(4932..4979)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(5046..5062)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"