Basic Vector Information
pWAS-anti-fadR-anti-xapR vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pWAS-anti-fadR-anti-xapR vector Sequence
LOCUS 40924_46173 5192 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pWAS-anti-fadR-anti-xapR, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5192)
AUTHORS Kim B, Binkley R, Kim HU, Lee SY.
TITLE Metabolic engineering of Escherichia coli for the enhanced
production of l-tyrosine
JOURNAL Biotechnol. Bioeng. (2018) In press
PUBMED 30019750
REFERENCE 2 (bases 1 to 5192)
AUTHORS Yang D, Kim WJ, Yoo SM, Choi JH, Ha SH, Lee MH, Lee SY.
TITLE Direct Submission
JOURNAL Submitted (15-JUN-2018) Dept. Chemical and Biomolecular Engineering,
Korea Advanced Institute of Science and Technology, Daehak-ro 291,
Daejeon 34141, South Korea
REFERENCE 3 (bases 1 to 5192)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5192)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
Bioeng. (2018) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-JUN-2018) Dept. Chemical and Biomolecular Engineering, Korea
Advanced Institute of Science and Technology, Daehak-ro 291, Daejeon
34141, South Korea"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5192
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 292..880
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 1123..1150
/label=T7Te terminator
/note="phage T7 early transcription terminator"
terminator 1191..1234
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ncRNA 1252..1330
/product="micC"
/label=antisense_RNA
/ncRNA_class="antisense_RNA"
misc_binding complement(1331..1354)
/label=anti-fadR binding site
/bound_moiety="anti-fadR"
regulatory complement(1355..1403)
/label=lambda PR promoter
/note="lambda PR promoter"
/regulatory_class="promoter"
primer_bind complement(1423..1439)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
terminator complement(1446..1473)
/label=T7Te terminator
/note="phage T7 early transcription terminator"
terminator complement(1489..1560)
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS complement(1578..2288)
/label=lambda repressor
/note="phage lambda repressor"
promoter complement(2331..2615)
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
CDS 2642..3517
/label=araC
/note="L-arabinose regulatory protein"
regulatory 3609..3657
/label=lambda PR promoter
/note="lambda PR promoter"
/regulatory_class="promoter"
misc_binding 3658..3681
/label=anti-xapR binding site
/bound_moiety="anti-xapR"
ncRNA 3682..3760
/product="micC"
/label=antisense_RNA
/ncRNA_class="antisense_RNA"
terminator 3775..3846
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3862..3889
/label=T7Te terminator
/note="phage T7 early transcription terminator"
promoter 4049..4153
/label=AmpR promoter
CDS 4154..5011
/label=AmpR
/note="beta-lactamase"
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