pVR37 vector (V002226)

Basic Vector Information

      • Vector Name:
      • pVR37
      • Antibiotic Resistance:
      • Hygromycin
      • Length:
      • 12588 bp
      • Type:
      • Promoter probe vector
      • Source/Author:
      • Medi VG, Bhat RS, Kuruvinashetti MS.

pVR37 vector Vector Map

pVR3712588 bp60012001800240030003600420048005400600066007200780084009000960010200108001140012000M13 fwdCaMV 35S promotercat1 intron6xHisNOS terminatorRB T-DNA repeatpVS1 StaApVS1 RepApVS1 oriVbomoriKanRLB T-DNA repeatCaMV poly(A) signalHygRCaMV 35S promoter (enhanced)CAP binding sitelac promoterlac operatorM13 revxylanase A5'UTR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pVR37 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_46108       12588 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pVR37, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Medi VG, Bhat RS, Kuruvinashetti MS.
  TITLE     Promoter-probe vector with synthetic xylanase reporter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12588)
  AUTHORS   Medi VG, Bhat RS, Kuruvinashetti MS.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2008) Department of Biotechnology, University of 
            Agricultural Sciences, Dharwad, Karnataka 580 005, India
REFERENCE   3  (bases 1 to 12588)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12588)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAY-2008) Department of Biotechnology, University of 
            Agricultural Sciences, Dharwad, Karnataka 580 005, India"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..55)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        432..777
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     intron          809..998
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             993..2816
                     /label=GUSPlus(TM)
                     /note="beta-glucuronidase"
     CDS             2823..2840
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      2872..3124
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3146..3170
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4470..5096
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             5533..6597
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      6666..6860
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7204..7344
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7530..8118)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8208..8999)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    9424..9448
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9526..9700)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9743..10765)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(10833..11510)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11701..11722
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11737..11767
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11775..11791
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11799..11815
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(11846..12559)
                     /codon_start=1
                     /product="xylanase A"
                     /label=xylanase A
                     /note="derived from Neocallimastix patriciarum"
                     /protein_id="ACE77059.1"
                     /translation="MASNGKKFTVGNGQNQHKGVNDGFSYEIWLDNTGGNGSMTLGSGA
                     TFKAEWNAAVNRGNFLARRGLDFGSQKKATDYDYIGLDYAATYKQTASASGNSRLCVYG
                     WFQNRGLNGVPLVEYYIIEDWVDWVPDAQGKMVTIDGAQYKIFQMDHTGPTINGGSETF
                     KQYFSVRQQKRTSGHITVSDHFKEWAKQGWGIGNLYEVALNAEGWQSSGVADVTLLDVY
                     TTPKGSSPATSAAPR"
     5'UTR           complement(12560..12587)

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