Basic Vector Information
pVMGCRT85 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVMGCRT85 vector Sequence
LOCUS 40924_46043 11368 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pVMGCRT85, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11368)
AUTHORS Gao M, Teplitski M.
TITLE RIVET-a tool for in vivo analysis of symbiotically relevant gene
expression in Sinorhizobium meliloti
JOURNAL Mol. Plant Microbe Interact. 21 (2), 162-170 (2008)
PUBMED 18184060
REFERENCE 2 (bases 1 to 11368)
AUTHORS Gao M, Teplitski M.
TITLE Direct Submission
JOURNAL Submitted (18-OCT-2007) Soil and Water Science, University of
REFERENCE 3 (bases 1 to 11368)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 11368)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant
Microbe Interact."; date: "2008"; volume: "21"; issue: "2"; pages:
"162-170"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(18-OCT-2007) Soil and Water Science, University of Florida-IFAS,
1376 Mowry Rd., Cancer "
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..11368
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 28..58
/label=Escherichia coli trpA
/note="Escherichia coli trpA"
/regulatory_class="terminator"
misc_feature 90..612
/note="partial sequence of Sinorhizobium meliloti nodB
(Sma0868) and nodC (Sma0866)"
misc_feature 624..635
/label=contains stop codons in all three reading frames
/note="contains stop codons in all three reading frames"
regulatory 711..717
/gene="tnpR"
/regulatory_class="ribosome_binding_site"
CDS 727..1275
/gene="tnpR"
/label=tnpR
/note="Transposon gamma-delta resolvase from Escherichia
coli (strain K12). Accession#: P03012"
regulatory 1278..1283
/gene="uidA"
/regulatory_class="ribosome_binding_site"
CDS 1310..3118
/label=GUS
/note="beta-glucuronidase"
CDS 3541..4332
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
promoter 5345..5373
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 5421..6608
/label=TcR
/note="tetracycline efflux protein"
promoter 7735..7763
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(8287..8303)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 8516..8971
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
oriT complement(9310..9419)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
promoter 9589..9693
/label=AmpR promoter
CDS 9694..10551
/label=AmpR
/note="beta-lactamase"
rep_origin 10725..11313
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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