Basic Vector Information
- Vector Name:
- pVMExp14367
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6093 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Verma V, Kaur C, Grover P, Gupta A, Chaudhary VK.
- Promoter:
- sacB
pVMExp14367 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVMExp14367 vector Sequence
LOCUS 40924_46033 6093 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pVMExp14367, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6093)
AUTHORS Verma V, Kaur C, Grover P, Gupta A, Chaudhary VK.
TITLE Biotin-tagged proteins: Reagents for efficient ELISA-based
serodiagnosis and phage display-based affinity selection
JOURNAL PLoS ONE 13 (1), e0191315 (2018)
PUBMED 29360877
REFERENCE 2 (bases 1 to 6093)
AUTHORS Chaudhary VK, Gupta A, Verma V, Kaur C, Grover P.
TITLE Direct Submission
JOURNAL Submitted (02-DEC-2017) Centre for Innovation in Infectious Disease
Research, Education, and Training, University of Delhi South Campus,
Benito Juarez Road, New Delhi, New Delhi 110021, India
REFERENCE 3 (bases 1 to 6093)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6093)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
date: "2018"; volume: "13"; issue: "1"; pages: "e0191315"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-DEC-2017) Centre for Innovation in Infectious Disease Research,
Education, and Training, University of Delhi South Campus, Benito
Juarez Road, New Delhi, New Delhi 110021, India"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6093
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 20..44
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 59..81
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 98..124
/codon_start=1
/label=9xHis
/note="9xHis affinity tag"
/translation="HHHHHHHHH"
CDS 143..163
/codon_start=1
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
/translation="ENLYFQG"
misc_feature complement(169..179)
/label=BsaI-5' cloning site
/note="BsaI-5' cloning site"
promoter 180..625
/label=sacB promoter
/note="sacB promoter and control region"
CDS 626..2044
/codon_start=1
/label=SacB
/note="secreted levansucrase that renders bacterial growth
sensitive to sucrose"
/translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
VKDSILEQGQLTVNK"
misc_feature 2084..2094
/label=BsaI-3' cloning site
/note="BsaI-3' cloning site"
misc_feature 2091..2117
/label=spacer
/note="spacer"
CDS 2118..2162
/codon_start=1
/label=AviTag(TM)
/note="peptide tag that allows for enzymatic biotinylation"
/translation="GLNDIFEAQKIEWHE"
terminator 2230..2277
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 2314..2769
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2796..2900
/label=AmpR promoter
CDS 2901..3758
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3932..4520
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind complement(4591..4612)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(4628..5707)
/codon_start=1
/label=lacI
/note="lac repressor"
/translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(5708..5785)
/label=lacI promoter
This page is informational only.