pVC7N vector (V002251)

Basic Vector Information

      • Vector Name:
      • pVC7N
      • Antibiotic Resistance:
      • Chloramphenicol
      • Length:
      • 6679 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Hashiro S, Yasueda H.

pVC7N vector Vector Map

pVC7N6679 bp3006009001200150018002100240027003000330036003900420045004800510054005700600063006600pAM330N regionCmRCAP binding sitelac promoterlac operatorM13 revMCSM13 fwdori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pVC7N vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45903        6679 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVC7N DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   Hashiro S, Yasueda H.
  TITLE     High copy number mutants derived from Corynebacterium glutamicum 
            cryptic plasmid pAM330 and copy number control
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6679)
  AUTHORS   Hashiro S, Yasueda H.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-SEP-2018) Contact:Shuhei Hashiro AJINOMOTO CO., INC., 
            Frontier Research Labs. Institute For Innovation; 1-1 Suzuki-Cho, 
            Kawasaki-Ku, Kawasaki, Kanagawa 210-8681, Japan URL 
            :http://www.ajinomoto.com/
REFERENCE   3  (bases 1 to 6679)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6679)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-SEP-2018) Contact:Shuhei Hashiro AJINOMOTO CO., INC., Frontier 
            Research Labs. Institute For Innovation; 1-1 Suzuki-Cho, 
            Kawasaki-Ku, Kawasaki, Kanagawa 210-8681, Japan URL 
            :http://www.ajinomoto.com/"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..4451
                     /label=pAM330N region
                     /note="pAM330N region"
     CDS             1215..2600
                     /codon_start=1
                     /gene="repA"
                     /label=repA
                     /protein_id="BBG56731.1"
                     /translation="MTTIALAKTTGTTPAQTRTAQPRTTSGLGNTEIEVNTSKEPQVNE
                     GSKVTRARAWRRQNVMYKITNSKALAGCHRWRRDEAVAVSWSSNGASQFEGLQNSHSRW
                     GSPLAELEVMGERRIELAIATKNHLAAGGALMMFVGTVRHNRSQSFAQVEAGIKTAYSS
                     MVKTSQWKKERARYGVEHTYSDYEVTDSWANGWHLHRNMLLFLDRPLSDDELKAFEDSM
                     FSRWSAGVVKAGMDAPLREHGVKLDQVSTWGGDAAKMATYLAKGMSQELTGSATKTASK
                     GSYTPFQMLDMLADQSDAGEDMDAVLVARWREYEVGSKNLRSSWSRGAKRALGIDYIDA
                     DVRREMEEELYKLAGLEAPERVESTRVAVALVKPDDWKLIQSDFAVRQYVLDCVDKAKD
                     VAAAQRVANEVLASLGVDSTPCMIVMDDVDLDAVLPTHGDATKRDLNAAVFAGNEQTIL
                     RTH"
     gene            1215..2600
                     /gene="repA"
                     /label=repA
     CDS             4545..5201
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     protein_bind    5413..5434
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5449..5479
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5487..5503
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5511..5527
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(5540..5596)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5597..5613)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(5939..6527)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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