pUNIV vector (V002276)

LOCUS       40924_45778        5653 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pUNIV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson 
            KR, Boileau AJ.
  TITLE     Optimized expression vector for ion channel studies in Xenopus 
            oocytes and mammalian cells using alfalfa mosaic virus
  JOURNAL   Pflugers Arch. 454 (1), 155-163 (2007)
  PUBMED    17146677
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Boileau AJ, Venkatachalan SP, Bushman JD, Mercado JL.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-DEC-2006) Physiology, Univ. of Wisconsin-Madison, 601 
            Science Dr., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5653)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5653)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pflugers 
            Arch."; date: "2007"; volume: "454"; issue: "1"; pages: "155-163"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-DEC-2006) Physiology, Univ. of Wisconsin-Madison, 601 Science 
            Dr., Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1085..1107
                     /label=modified multiple cloning region
                     /note="modified multiple cloning region"
     3'UTR           1124..1249
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     regulatory      complement(1321..1339)
                     /label=T3 promoter
                     /note="T3 promoter"
                     /regulatory_class="promoter"
     promoter        complement(1322..1339)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(1357..1478)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1664..2119
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2236..2593
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2644..3435
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3502..3550
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        3856..3960
                     /label=AmpR promoter
     CDS             3961..4818
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4992..5580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"