Basic Vector Information
- Vector Name:
- pUNIV
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5653 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson KR, Boileau AJ.
- Promoter:
- CMV
pUNIV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUNIV vector Sequence
LOCUS 40924_45778 5653 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pUNIV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5653)
AUTHORS Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson
KR, Boileau AJ.
TITLE Optimized expression vector for ion channel studies in Xenopus
oocytes and mammalian cells using alfalfa mosaic virus
JOURNAL Pflugers Arch. 454 (1), 155-163 (2007)
PUBMED 17146677
REFERENCE 2 (bases 1 to 5653)
AUTHORS Boileau AJ, Venkatachalan SP, Bushman JD, Mercado JL.
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2006) Physiology, Univ. of Wisconsin-Madison, 601
Science Dr., Madison, WI 53711, USA
REFERENCE 3 (bases 1 to 5653)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5653)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Pflugers
Arch."; date: "2007"; volume: "454"; issue: "1"; pages: "155-163"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-DEC-2006) Physiology, Univ. of Wisconsin-Madison, 601 Science
Dr., Madison, WI 53711, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5653
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 139..517
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 518..729
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 890..1022
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
promoter 1067..1085
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 1085..1107
/label=modified multiple cloning region
/note="modified multiple cloning region"
3'UTR 1124..1249
/label=Xenopus globin 3'-UTR
/note="3'-UTR of the major beta-globin gene of Xenopus
laevis"
regulatory complement(1321..1339)
/label=T3 promoter
/note="T3 promoter"
/regulatory_class="promoter"
promoter complement(1322..1339)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal complement(1357..1478)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 1664..2119
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2236..2593
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2644..3435
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 3502..3550
/label=poly(A) signal
/note="synthetic polyadenylation signal"
promoter 3856..3960
/label=AmpR promoter
CDS 3961..4818
/label=AmpR
/note="beta-lactamase"
rep_origin 4992..5580
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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