pUltraBac-1 vector (V002287)

MSDS

Basic Vector Information

Vector Name:: pUltraBac-1

Antibiotic Resistance:: Ampicillin

Length:: 6159 bp

Type:: Baculovirus expression vector

Replication origin:: ori

Source/Author:: Philipps B, Rotmann D, Wicki M, Mayr LM, Forstner M.

Promoter:: basic protein

pUltraBac-1 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUltraBac-1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45718        6159 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pUltraBac-1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Philipps B, Rotmann D, Wicki M, Mayr LM, Forstner M.
  TITLE     Time reduction and process optimization of the baculovirus 
            expression system for more efficient recombinant protein production 
            in insect cells
  JOURNAL   Protein Expr. Purif. 42 (1), 211-218 (2005)
  PUBMED    15939308
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Philipps B, Rotmann D, Wicki M, Mayr LM, Forstner M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2005) Novartis Institutes for BioMedical Research,
            Lead Discovery Center, WSJ-88.630, Basel CH-4002, Switzerland
REFERENCE   3  (bases 1 to 6159)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6159)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2005"; volume: "42"; issue: "1"; pages: 
            "211-218"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-APR-2005) Novartis Institutes for BioMedical Research, Lead 
            Discovery Center, WSJ-88.630, Basel CH-4002, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      102..557
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        584..688
                     /label=AmpR promoter
     CDS             689..1546
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1720..2308
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2611..2835)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2905..3435)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3624..3652)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     polyA_signal    complement(4161..4209)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(4304..5020)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(5038..5362)
                     /label=basic protein promoter
                     /note="baculovirus basic protein promoter, which allows 
                     expression during the late instead of very late phase of 
                     viral infection"
     promoter        5396..5487
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5749..5883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(5912..6077)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"

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