Price Information
Cat No. | Plasmid Name | Availability | Add to cart |
---|---|---|---|
V002331 | pUG6 | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
Plasmid pUG6 carrying the loxP–kanMX–loxP module. LoxP-flanked marker gene deletion cassette: loxP-pAgTEF1-kanMX-tAgTEF1-loxP, selectable phenotype: G418 resistance
pUG6 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
References
- Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH. A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast. Nucleic Acids Res. 2002 Mar 15;30(6):e23.
pUG6 vector Sequence
LOCUS 40924_45503 4009 bp DNA circular SYN 18-DEC-2018 DEFINITION PCR template vector pUG6, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4009) AUTHORS Guldener U, Heck S, Fielder T, Beinhauer J, Hegemann JH. TITLE A new efficient gene disruption cassette for repeated use in budding yeast JOURNAL Nucleic Acids Res. 24 (13), 2519-2524 (1996) PUBMED 8692690 REFERENCE 2 (bases 1 to 4009) AUTHORS Gueldener U, Heinisch J, Koehler GJ, Voss D, Hegemann JH. TITLE A second set of loxP marker cassettes for Cre-mediated multiple gene knockouts in budding yeast JOURNAL Nucleic Acids Res. 30 (6), E23 (2002) PUBMED 11884642 REFERENCE 3 (bases 1 to 4009) AUTHORS Gueldener U, Hegemann JH, Heck S, Fiedler T, Beinhauer JD. TITLE Direct Submission JOURNAL Submitted (23-AUG-2000) Institut fuer Mikrobiologie, Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf 40225, Germany REFERENCE 4 (bases 1 to 4009) TITLE Direct Submission REFERENCE 5 (bases 1 to 4009) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "1996"; volume: "24"; issue: "13"; pages: "2519-2524" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "2002"; volume: "30"; issue: "6"; pages: "E23" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (23-AUG-2000) Institut fuer Mikrobiologie, Heinrich-Heine-Universitaet, Universtitaetsstr. 1, Duesseldorf 40225, Germany" COMMENT SGRef: number: 4; type: "Journal Article" FEATURES Location/Qualifiers source 1..4009 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 53..86 /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." gene 141..1497 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" misc_feature 1560..1593 /label=loxP site /note="loxP site" protein_bind complement(1560..1593) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." promoter complement(1647..1665) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(1923..2511) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2685..3542) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(3543..3647) /label=AmpR promoter promoter 3993..4009 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"