Basic Vector Information
- Vector Name:
- pUDI069
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6697 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D, van Maris AJ, Pronk JT, Daran JM.
- Promoter:
- GPD
pUDI069 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUDI069 vector Sequence
LOCUS 40924_45468 6697 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pUDI069, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6697)
AUTHORS Koopman F, Beekwilder J, Crimi B, Houwelingen A, Hall RD, Bosch D,
van Maris AJ, Pronk JT, Daran JM.
TITLE De novo production of the flavonoid naringenin in engineered
Saccharomyces cerevisiae
JOURNAL Microb. Cell Fact. 11 (1), 155 (2012)
PUBMED 23216753
REFERENCE 2 (bases 1 to 6697)
AUTHORS Koopman F, Beekwilder J, Crimi B, van Houwelingen A, Hall RD, Bosch
D, Maris AJA., Pronk JT, Daran J-M.
TITLE Direct Submission
JOURNAL Submitted (02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600,
The Netherlands
REFERENCE 3 (bases 1 to 6697)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6697)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microb.
Cell Fact."; date: "2012"; volume: "11"; issue: "1"; pages: "155"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(02-JUL-2012) Platform Green Synthetic Biolog, Delft 2600, The
Netherlands"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6697
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(148..736)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(910..1767)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1768..1872)
/label=AmpR promoter
promoter 2152..2432
/label=TRP1 promoter
CDS 2433..3104
/codon_start=1
/label=TRP1
/note="phosphoribosylanthranilate isomerase, required for
tryptophan biosynthesis"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
rep_origin complement(3204..3659)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 3804..3820
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3827..3845
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 3870..4514
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase; also known as the TDH3 promoter"
CDS 4532..6127
/codon_start=1
/product="tyrosine ammonia lyase"
/label=tyrosine ammonia lyase
/note="Tal1"
/protein_id="AGA15802.1"
/translation="MTLQSQTAKDCLALDGALTLVQCEAIATHRSRISVTPALRERCAR
AHARLEHAIAEQRHIYGITTGFGPLANRLIGADQGAELQQNLIYHLATGVGPKLSWAEA
RALMLARLNSILQGASGASPETIDRIVAVLNAGFAPEVPAQGTVGASGDLTPLAHMVLA
LQGRGRMIDPSGRVQEAGAVMDRLCGGPLTLAARDGLALVNGTSAMTAIAALTGVEAAR
AIDAALRHSAVLMEVLSGHAEAWHPAFAELRPHPGQLRATERLAQALDGAGRVCRTLTA
ARRLTAADLRPEDHPAQDAYSLRVVPQLVGAVWDTLDWHDRVVTCELNSVTDNPIFPEG
CAVPALHGGNFMGVHVALASDALNAALVTLAGLVERQIARLTDEKLNKGLPAFLHGGQA
GLQSGFMGAQVTATALLAEMRANATPVSVQSLSTNGANQDVVSMGTIAARRARAQLLPL
SQIQAILALALAQAMDLLDDPEGQAGWSLTARDLRDRIRAVSPGLRADRPLAGHIEAVA
QGLRHPSAAADPPA"
terminator 6134..6381
/label=CYC1 terminator
/note="transcription terminator for CYC1"
promoter complement(6404..6422)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6443..6459)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6467..6483)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6491..6521)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6536..6557)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
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