Basic Vector Information
- Vector Name:
- pUCP18T-miniTN7-lux-Gm
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11888 bp
- Type:
- Reporter vector
- Replication origin:
- ori
- Source/Author:
- Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, Goldberg JB.
- Promoter:
- Pc
pUCP18T-miniTN7-lux-Gm vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUCP18T-miniTN7-lux-Gm vector Sequence
LOCUS 40924_45293 11888 bp DNA circular SYN 18-DEC-2018 DEFINITION Reporter vector pUCP18T-miniTN7-lux-Gm, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11888) AUTHORS Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, Goldberg JB. TITLE Construction of conjugatable Mini-Tn7 Vectors for Bioluminescent Detection and Single Copy Promoter lux Reporter Analysis in Gram-negative Bacteria JOURNAL Unpublished REFERENCE 2 (bases 1 to 11888) AUTHORS Damron FH, McKenney ES, Barbier M, Liechti GW, Schweizer HP, Goldberg JB. TITLE Direct Submission JOURNAL Submitted (30-MAR-2013) Microbiology, Immunology, and Cancer Biology, University of Virginia, PO Box 800734, Charlottesville, VA 22908, USA REFERENCE 3 (bases 1 to 11888) TITLE Direct Submission REFERENCE 4 (bases 1 to 11888) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (30-MAR-2013) Microbiology, Immunology, and Cancer Biology, University of Virginia, PO Box 800734, Charlottesville, VA 22908, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..11888 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 349..365 /label=KS primer /note="common sequencing primer, one of multiple similar variants" terminator 393..487 /label=lambda t0 terminator /note="transcription terminator from phage lambda" terminator 590..676 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" misc_feature 722..749 /label=FRT /note="FRT" CDS complement(835..1365) /label=GmR /note="gentamycin acetyltransferase" regulatory 1424..1465 /label=promoter 2 /note="promoter 2" /regulatory_class="promoter" promoter complement(1554..1582) /label=Pc promoter /note="class 1 integron promoter" protein_bind 1623..1670 /label=FRT /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." promoter 1766..1794 /label=Pc promoter /note="class 1 integron promoter" primer_bind complement(2191..2213) /label=Plux seq R /note="Plux seq R" CDS 2641..4080 /label=LuxC /note="LuxC fatty acid reductase" CDS 4095..5015 /label=LuxD /note="LuxD acyltransferase" CDS 5067..6146 /label=LuxA /note="LuxA luciferase subunit" CDS 6164..7144 /label=LuxB /note="LuxB luciferase subunit" CDS 7326..8435 /label=LuxE /note="LuxE" mobile_element complement(9169..9334) /label=Tn7L /note="mini-Tn7 element (left end of the Tn7 transposon)" oriT complement(9493..9601) /direction=LEFT /label=oriT /note="incP origin of transfer" rep_origin complement(9833..10421) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(10595..11452) /label=AmpR /note="beta-lactamase" promoter complement(11453..11557) /label=AmpR promoter
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