Basic Vector Information
pUC57-Bt-pheS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC57-Bt-pheS vector Sequence
LOCUS 40924_45168 3816 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUC57-Bt-pheS, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3816) AUTHORS Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT. TITLE Genetic tools for allelic replacement in Burkholderia species JOURNAL Appl. Environ. Microbiol. 74 (14), 4498-4508 (2008) PUBMED 18502918 REFERENCE 2 (bases 1 to 3816) AUTHORS Barrett AR, Kang Y, Son MS, Vukovich JM, Hoang TT. TITLE Direct Submission JOURNAL Submitted (05-NOV-2007) Microbiology, University of Hawaii Manoa, 2538 McCarthy Mall Snyder Hall, Rm. 310, Honolulu, HI 96822, USA REFERENCE 3 (bases 1 to 3816) TITLE Direct Submission REFERENCE 4 (bases 1 to 3816) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl. Environ. Microbiol."; date: "2008"; volume: "74"; issue: "14"; pages: "4498-4508" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (05-NOV-2007) Microbiology, University of Hawaii Manoa, 2538 McCarthy Mall Snyder Hall, Rm. 310, Honolulu, HI 96822, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..3816 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" CDS complement(444..1454) /gene="pheS" /label=pheS /note="Phenylalanine--tRNA ligase alpha subunit from Burkholderia pseudomallei (strain 1710b). Accession#: Q3JT08" primer_bind complement(1595..1611) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(1619..1635) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1643..1673) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(1688..1709) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(1997..2585) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2759..3616) /label=AmpR /note="beta-lactamase" promoter complement(3617..3721) /label=AmpR promoter
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