pUC57-Bt-pheS vector (V002393)

Basic Vector Information

      • Vector Name:
      • pUC57-Bt-pheS
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3816 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT.

pUC57-Bt-pheS vector Vector Map

pUC57-Bt-pheS3816 bp60012001800240030003600M13 fwdpheSM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pUC57-Bt-pheS vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_45168        3816 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC57-Bt-pheS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Barrett AR, Kang Y, Inamasu KS, Son MS, Vukovich JM, Hoang TT.
  TITLE     Genetic tools for allelic replacement in Burkholderia species
  JOURNAL   Appl. Environ. Microbiol. 74 (14), 4498-4508 (2008)
  PUBMED    18502918
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Barrett AR, Kang Y, Son MS, Vukovich JM, Hoang TT.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2007) Microbiology, University of Hawaii Manoa, 
            2538 McCarthy Mall Snyder Hall, Rm. 310, Honolulu, HI 96822, USA
REFERENCE   3  (bases 1 to 3816)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2008"; volume: "74"; issue: "14"; 
            pages: "4498-4508"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-NOV-2007) Microbiology, University of Hawaii Manoa, 2538 
            McCarthy Mall Snyder Hall, Rm. 310, Honolulu, HI 96822, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(444..1454)
                     /gene="pheS"
                     /label=pheS
                     /note="Phenylalanine--tRNA ligase alpha subunit from 
                     Burkholderia pseudomallei (strain 1710b). Accession#: 
                     Q3JT08"
     primer_bind     complement(1595..1611)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1619..1635)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1643..1673)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1688..1709)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1997..2585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2759..3616)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3617..3721)
                     /label=AmpR promoter

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