Basic Vector Information
- Vector Name:
- pUC21
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3200 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Vieira J, Messing J.
pUC21 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC21 vector Sequence
LOCUS 40924_45158 3200 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUC21, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3200)
AUTHORS Vieira J, Messing J.
TITLE New pUC-derived cloning vectors with different selectable markers
and DNA replication origins
JOURNAL Gene 100, 189-194 (1991)
PUBMED 1905257
REFERENCE 2 (bases 1 to 3200)
AUTHORS Vieira J, Messing J.
TITLE Direct Submission
JOURNAL Submitted (12-JAN-2000) Waksman Institute, Rutgers University, 190
Frelinghuysen Road, Piscataway, NJ 08854, USA
REFERENCE 3 (bases 1 to 3200)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3200)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene 100,
189-194 (1991)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-JAN-2000) Waksman Institute, Rutgers University, 190
Frelinghuysen Road, Piscataway, NJ 08854, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3200
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 107..128
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
protein_bind 183..199
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 207..223
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 351..367
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(382..400)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(407..423)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 1295..1399
/label=AmpR promoter
CDS 1400..2257
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2431..3019
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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