Basic Vector Information
- Vector Name:
- pUC19TpTer
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3532 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira T, Bull EH, Butt AT, Thomas MS.
- Promoter:
- Pc
pUC19TpTer vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC19TpTer vector Sequence
LOCUS 40924_45148 3532 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pUC19TpTer, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3532)
AUTHORS Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira
T, Bull EH, Butt AT, Thomas MS.
TITLE An efficient system for the generation of marked genetic mutants in
members of the genus Burkholderia
JOURNAL Plasmid (2016) In press
PUBMED 27825973
REFERENCE 2 (bases 1 to 3532)
AUTHORS Shastri S, Spiewak HL, Sofoluwe A, Eidsvaag VA, Asghar AH, Pereira
T, Bull EH, Thomas MS.
TITLE Direct Submission
JOURNAL
REFERENCE 3 (bases 1 to 3532)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3532)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid
(2016) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-JUL-2016) Department of Infection, Immunity "
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3532
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 425..453
/label=Pc promoter
/note="class 1 integron promoter"
CDS 779..1012
/codon_start=1
/label=TpR
/note="E. coli plasmid-associated dihydrofolate reductase"
/translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
terminator 1039..1125
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 1217..1244
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
primer_bind complement(1311..1327)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1335..1351)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1359..1389)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1404..1425)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1713..2301)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2475..3332)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3333..3437)
/label=AmpR promoter
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