Basic Vector Information
- Vector Name:
- pUC18_LexA+Cp190
- Antibiotic Resistance:
- Ampicillin
- Length:
- 12082 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, Belyaeva ES, Belyakin SN.
- Promoter:
- hsp70
pUC18_LexA+Cp190 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUC18_LexA+Cp190 vector Sequence
LOCUS 40924_45128 12082 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pUC18_LexA+Cp190, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12082) AUTHORS Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, Belyaeva ES, Belyakin SN. TITLE Repressive Histone Marks Renewal Impedes Replication Fork Progression and Contributes To Late Replication in Drosophila JOURNAL Unpublished REFERENCE 2 (bases 1 to 12082) AUTHORS Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, Belyaeva ES, Belyakin SN. TITLE Direct Submission JOURNAL Submitted (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave. 8/2, Novosibirsk, NSO 630090, Russia REFERENCE 3 (bases 1 to 12082) TITLE Direct Submission REFERENCE 4 (bases 1 to 12082) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (03-JUN-2016) Genomics lab, IMCB SD RAS, Lavrentjeva ave. 8/2, Novosibirsk, NSO 630090, Russia" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..12082 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind 220..289 /label=attB /note="attB site for the phi-C31 integrase (Groth et al., 2000)" protein_bind 534..567 /label=FRT (minimal) /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" promoter 1448..1900 /label=hsp70 promoter /note="Drosophila melanogaster hsp70Bb promoter" primer_bind complement(1970..1986) /label=KS primer /note="common sequencing primer, one of multiple similar variants" CDS 2004..2609 /label=LexA /note="E. coli DNA-binding protein that represses genes involved in the SOS response to DNA damage" CDS 2619..2639 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" CDS 2652..5939 /gene="Cp190" /label=Cp190 /note="Centrosome-associated zinc finger protein Cp190 from Drosophila melanogaster. Accession#: Q24478" protein_bind 10161..10194 /label=FRT (minimal) /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" rep_origin complement(10263..10851) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(11025..11882) /label=AmpR /note="beta-lactamase" promoter complement(11883..11987) /label=AmpR promoter
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